Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 2508753..2509731 Replicon chromosome
Accession NZ_CP014847
Organism Bacillus thuringiensis strain HD12

Toxin (Protein)


Gene name spoIISA Uniprot ID B7IX38
Locus tag A3L20_RS12880 Protein ID WP_000624981.1
Coordinates 2508753..2509490 (+) Length 246 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID B7IX39
Locus tag A3L20_RS12885 Protein ID WP_000237817.1
Coordinates 2509603..2509731 (+) Length 43 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A3L20_RS12860 2504398..2505180 + 783 WP_016077713.1 class I SAM-dependent methyltransferase -
A3L20_RS12865 2505338..2507014 - 1677 Protein_2509 alpha-keto acid decarboxylase family protein -
A3L20_RS12870 2507129..2507611 + 483 WP_000191905.1 MarR family winged helix-turn-helix transcriptional regulator -
A3L20_RS12875 2507777..2508514 + 738 WP_000594159.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
A3L20_RS12880 2508753..2509490 + 738 WP_000624981.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
A3L20_RS12885 2509603..2509731 + 129 WP_000237817.1 hypothetical protein Antitoxin
A3L20_RS12890 2509804..2509980 + 177 WP_000852629.1 stage II sporulation protein SB -
A3L20_RS12895 2509999..2510388 - 390 WP_000712655.1 YxeA family protein -
A3L20_RS12900 2510600..2512069 + 1470 WP_000287540.1 beta-Ala-His dipeptidase -
A3L20_RS12905 2512382..2513191 + 810 WP_016077711.1 C39 family peptidase -
A3L20_RS12910 2513217..2513822 + 606 WP_000517276.1 hypothetical protein -
A3L20_RS12915 2513963..2514214 - 252 WP_000821942.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(10-243)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 246 a.a.        Molecular weight: 28335.09 Da        Isoelectric Point: 8.2998

>T61842 WP_000624981.1 NZ_CP014847:2508753-2509490 [Bacillus thuringiensis]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNVFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Download         Length: 738 bp

>T61842 NZ_CP014847:2508753-2509490 [Bacillus thuringiensis]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACCACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAAGAATATACAAAGAGCTTAAATGTATTTTTAGATTCATATGGGGAAAAAATTGGCGCGAAAATT
GTTGTTTTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCAGTGCAAGTAGAAAATGTCGGGATCATACCAGCAAAAATAGTAAGTGATG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 43 a.a.        Molecular weight: 4981.37 Da        Isoelectric Point: 3.9150

>AT61842 WP_000237817.1 NZ_CP014847:2509603-2509731 [Bacillus thuringiensis]
VVKDYDNDFFKEDNEDTTDFSLIKGDALQQVEELEEELKRKS

Download         Length: 129 bp

>AT61842 NZ_CP014847:2509603-2509731 [Bacillus thuringiensis]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATGC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6D1SWF1

Antitoxin

Source ID Structure
AlphaFold DB B7IX39

References