Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 238608..239250 Replicon chromosome
Accession NZ_CP014847
Organism Bacillus thuringiensis strain HD12

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag A3L20_RS01335 Protein ID WP_000635965.1
Coordinates 238900..239250 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag A3L20_RS01330 Protein ID WP_000004570.1
Coordinates 238608..238895 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A3L20_RS01305 233925..234887 + 963 WP_016078970.1 UV DNA damage repair endonuclease UvsE -
A3L20_RS01310 234880..235452 - 573 WP_016078971.1 rhomboid family intramembrane serine protease -
A3L20_RS01315 235546..235905 + 360 WP_000583417.1 holo-ACP synthase -
A3L20_RS01320 236062..237012 + 951 WP_062804426.1 outer membrane lipoprotein carrier protein LolA -
A3L20_RS01325 237130..238299 + 1170 WP_000390595.1 alanine racemase -
A3L20_RS01330 238608..238895 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
A3L20_RS01335 238900..239250 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
A3L20_RS01340 239318..241486 + 2169 WP_016078972.1 RNA-binding transcriptional accessory protein -
A3L20_RS32715 241544..241660 - 117 WP_001143640.1 cortex morphogenetic protein CmpA -
A3L20_RS01345 241856..242314 + 459 WP_000343553.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T61841 WP_000635965.1 NZ_CP014847:238900-239250 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T61841 NZ_CP014847:238900-239250 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT61841 WP_000004570.1 NZ_CP014847:238608-238895 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT61841 NZ_CP014847:238608-238895 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTATATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCG
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References