Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3675988..3676624 Replicon chromosome
Accession NZ_CP014842
Organism Bacillus licheniformis strain SCDB 14

Toxin (Protein)


Gene name mazF Uniprot ID M5P3Q9
Locus tag B14_RS19025 Protein ID WP_003179128.1
Coordinates 3675988..3676338 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M5PDU2
Locus tag B14_RS19030 Protein ID WP_006638778.1
Coordinates 3676343..3676624 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B14_RS18985 3671029..3671628 - 600 WP_003179145.1 SpoIIE family protein phosphatase -
B14_RS18990 3671625..3672416 - 792 WP_003179144.1 RNA polymerase sigma factor SigB -
B14_RS18995 3672382..3672867 - 486 WP_003179142.1 anti-sigma B factor RsbW -
B14_RS19000 3672867..3673193 - 327 WP_003179140.1 anti-sigma factor antagonist -
B14_RS19005 3673252..3674259 - 1008 WP_003179137.1 PP2C family protein-serine/threonine phosphatase -
B14_RS19010 3674270..3674671 - 402 WP_003179135.1 anti-sigma regulatory factor -
B14_RS19015 3674674..3675039 - 366 WP_003179132.1 STAS domain-containing protein -
B14_RS19020 3675043..3675870 - 828 WP_003179130.1 RsbT co-antagonist protein RsbRA -
B14_RS19025 3675988..3676338 - 351 WP_003179128.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
B14_RS19030 3676343..3676624 - 282 WP_006638778.1 hypothetical protein Antitoxin
B14_RS19035 3676736..3677905 - 1170 WP_026080763.1 alanine racemase -
B14_RS19040 3678130..3679089 - 960 WP_152847888.1 outer membrane lipoprotein carrier protein LolA -
B14_RS19045 3679432..3680031 + 600 WP_003179118.1 rhomboid family intramembrane serine protease -
B14_RS19050 3680028..3681509 - 1482 WP_009330132.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12990.03 Da        Isoelectric Point: 5.7234

>T61840 WP_003179128.1 NZ_CP014842:c3676338-3675988 [Bacillus licheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Download         Length: 351 bp

>T61840 NZ_CP014842:c3676338-3675988 [Bacillus licheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGACTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10649.03 Da        Isoelectric Point: 4.6920

>AT61840 WP_006638778.1 NZ_CP014842:c3676624-3676343 [Bacillus licheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Download         Length: 282 bp

>AT61840 NZ_CP014842:c3676624-3676343 [Bacillus licheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTACGCGAACGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATCAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I7FHI4

Antitoxin

Source ID Structure
AlphaFold DB M5PDU2

References