Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3492352..3492988 Replicon chromosome
Accession NZ_CP014840
Organism Bacillus subtilis subsp. globigii strain ATCC 49760

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag A1D11_RS17175 Protein ID WP_003156187.1
Coordinates 3492352..3492702 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A837XXL9
Locus tag A1D11_RS17180 Protein ID WP_003328141.1
Coordinates 3492707..3492988 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A1D11_RS17135 3487404..3488003 - 600 WP_003328150.1 SpoIIE family protein phosphatase -
A1D11_RS17140 3488003..3488791 - 789 WP_003328149.1 RNA polymerase sigma factor SigB -
A1D11_RS17145 3488757..3489239 - 483 WP_061902497.1 anti-sigma B factor RsbW -
A1D11_RS17150 3489236..3489565 - 330 WP_003328146.1 anti-sigma factor antagonist RsbV -
A1D11_RS17155 3489625..3490632 - 1008 WP_003328145.1 PP2C family protein-serine/threonine phosphatase -
A1D11_RS17160 3490644..3491045 - 402 WP_003328144.1 serine/threonine-protein kinase RsbT -
A1D11_RS17165 3491048..3491413 - 366 WP_010789626.1 RsbT antagonist protein RsbS -
A1D11_RS17170 3491418..3492236 - 819 WP_003328142.1 STAS domain-containing protein -
A1D11_RS17175 3492352..3492702 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
A1D11_RS17180 3492707..3492988 - 282 WP_003328141.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
A1D11_RS17185 3493107..3494276 - 1170 WP_003328140.1 alanine racemase -
A1D11_RS17190 3494444..3495460 - 1017 WP_087941846.1 outer membrane lipoprotein carrier protein LolA -
A1D11_RS17195 3495628..3495993 - 366 WP_003328138.1 holo-ACP synthase -
A1D11_RS17200 3496093..3496692 + 600 WP_003328136.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T61836 WP_003156187.1 NZ_CP014840:c3492702-3492352 [Bacillus subtilis subsp. globigii]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T61836 NZ_CP014840:c3492702-3492352 [Bacillus subtilis subsp. globigii]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGAGTGCGCCCGGT
TTTGGTGATTCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGACTCCGTTATTTTGCTGGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATTAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10451.83 Da        Isoelectric Point: 5.1903

>AT61836 WP_003328141.1 NZ_CP014840:c3492988-3492707 [Bacillus subtilis subsp. globigii]
MSESSATTEMMISLPEALVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT61836 NZ_CP014840:c3492988-3492707 [Bacillus subtilis subsp. globigii]
TTGTCTGAATCCAGCGCAACAACCGAAATGATGATTAGCTTGCCGGAAGCACTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTAATATCACAAGCAGTGAGAGCCTATGTCAGCGAACGCACAACTCGTCACAACC
GTGATTTGATGAGACGCGGATATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAAGCTCATTTTGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB A0A837XXL9

References