Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | spoIISABC/SpoIISA-SpoIISB |
| Location | 2675225..2676141 | Replicon | chromosome |
| Accession | NZ_CP014840 | ||
| Organism | Bacillus subtilis subsp. globigii strain ATCC 49760 | ||
Toxin (Protein)
| Gene name | spoIISA | Uniprot ID | A0A7U5BWH7 |
| Locus tag | A1D11_RS13115 | Protein ID | WP_003327423.1 |
| Coordinates | 2675225..2675971 (+) | Length | 249 a.a. |
Antitoxin (Protein)
| Gene name | spoIISB | Uniprot ID | A0A7U5D589 |
| Locus tag | A1D11_RS13120 | Protein ID | WP_013390483.1 |
| Coordinates | 2675971..2676141 (+) | Length | 57 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| A1D11_RS13095 | 2670514..2671464 | - | 951 | WP_003327417.1 | ring-cleaving dioxygenase | - |
| A1D11_RS13100 | 2671884..2673200 | + | 1317 | WP_003327418.1 | amino acid permease | - |
| A1D11_RS13105 | 2673488..2674105 | + | 618 | WP_003327421.1 | DUF47 domain-containing protein | - |
| A1D11_RS13110 | 2674118..2675116 | + | 999 | WP_003327422.1 | inorganic phosphate transporter | - |
| A1D11_RS13115 | 2675225..2675971 | + | 747 | WP_003327423.1 | type II toxin-antitoxin system SpoIISA family toxin | Toxin |
| A1D11_RS13120 | 2675971..2676141 | + | 171 | WP_013390483.1 | type II toxin-antitoxin system SpoIISB family antitoxin | Antitoxin |
| A1D11_RS21400 | 2676239..2676361 | + | 123 | WP_010788356.1 | hypothetical protein | - |
| A1D11_RS13125 | 2676437..2677330 | - | 894 | WP_003327425.1 | N-acetylmuramoyl-L-alanine amidase | - |
| A1D11_RS13130 | 2677343..2677606 | - | 264 | WP_003327426.1 | phage holin | - |
| A1D11_RS13135 | 2677619..2677897 | - | 279 | WP_003327427.1 | hemolysin XhlA family protein | - |
| A1D11_RS13140 | 2677970..2678818 | - | 849 | WP_003327428.1 | hypothetical protein | - |
| A1D11_RS13145 | 2678894..2679058 | - | 165 | WP_003327430.1 | XkdX family protein | - |
| A1D11_RS13150 | 2679055..2679384 | - | 330 | WP_003327431.1 | XkdW family protein | - |
| A1D11_RS13155 | 2679401..2681140 | - | 1740 | WP_003327433.1 | pyocin knob domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 2665071..2708456 | 43385 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 249 a.a. Molecular weight: 29110.66 Da Isoelectric Point: 4.5650
>T61835 WP_003327423.1 NZ_CP014840:2675225-2675971 [Bacillus subtilis subsp. globigii]
MLLFFQIMVWCIMAALGLYVYAVWRFEAKVKEKMFAIRKTWYLIFVIGAMVYWTYDPSSLFREWTNYLIVAVSFALIDAF
IFLSAYVKKLAGNELETDTREILEENNEMLHVYLDKLKTYQYLLKNEPIHVYYGSIEAYAEGIDKLLKTYADKMNLTASL
CEYSTQLEKDRLTEHMDDAFEVQSRLDRKDVYYDQHGKLALIPFTVQTRSYVIKLASDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGDG
MLLFFQIMVWCIMAALGLYVYAVWRFEAKVKEKMFAIRKTWYLIFVIGAMVYWTYDPSSLFREWTNYLIVAVSFALIDAF
IFLSAYVKKLAGNELETDTREILEENNEMLHVYLDKLKTYQYLLKNEPIHVYYGSIEAYAEGIDKLLKTYADKMNLTASL
CEYSTQLEKDRLTEHMDDAFEVQSRLDRKDVYYDQHGKLALIPFTVQTRSYVIKLASDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGDG
Download Length: 747 bp
>T61835 NZ_CP014840:2675225-2675971 [Bacillus subtilis subsp. globigii]
ATGCTTTTATTCTTTCAGATTATGGTTTGGTGCATAATGGCCGCATTAGGCCTATACGTATATGCGGTGTGGCGGTTTGA
AGCAAAGGTGAAAGAAAAAATGTTTGCGATCCGGAAAACGTGGTATTTGATCTTTGTCATCGGTGCCATGGTATACTGGA
CGTATGACCCCTCTTCCCTTTTCAGGGAATGGACGAATTATTTGATTGTCGCTGTCAGCTTTGCTTTGATTGACGCCTTT
ATCTTTTTGAGCGCTTACGTAAAGAAACTTGCCGGCAATGAATTAGAAACCGACACACGGGAAATCCTTGAAGAAAACAA
TGAAATGCTCCACGTTTATCTTGATAAATTAAAAACGTACCAATACCTATTGAAAAATGAACCGATCCATGTTTATTATG
GAAGTATAGAAGCATACGCTGAGGGCATCGACAAGCTGCTCAAAACCTATGCCGACAAAATGAACTTAACGGCTTCGCTG
TGTGAATATTCGACACAGCTTGAAAAAGACCGCTTAACCGAGCATATGGATGATGCATTTGAGGTGCAGAGCCGGCTGGA
TCGAAAAGATGTATACTATGATCAGCACGGGAAGCTGGCACTCATCCCTTTTACAGTCCAGACACGCAGCTACGTCATCA
AGCTGGCGTCAGACAGCATTGTAACAGAATTTGATTATTTGCTTTTCACATCATTAACTAGCATTTACGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGACGGATAA
ATGCTTTTATTCTTTCAGATTATGGTTTGGTGCATAATGGCCGCATTAGGCCTATACGTATATGCGGTGTGGCGGTTTGA
AGCAAAGGTGAAAGAAAAAATGTTTGCGATCCGGAAAACGTGGTATTTGATCTTTGTCATCGGTGCCATGGTATACTGGA
CGTATGACCCCTCTTCCCTTTTCAGGGAATGGACGAATTATTTGATTGTCGCTGTCAGCTTTGCTTTGATTGACGCCTTT
ATCTTTTTGAGCGCTTACGTAAAGAAACTTGCCGGCAATGAATTAGAAACCGACACACGGGAAATCCTTGAAGAAAACAA
TGAAATGCTCCACGTTTATCTTGATAAATTAAAAACGTACCAATACCTATTGAAAAATGAACCGATCCATGTTTATTATG
GAAGTATAGAAGCATACGCTGAGGGCATCGACAAGCTGCTCAAAACCTATGCCGACAAAATGAACTTAACGGCTTCGCTG
TGTGAATATTCGACACAGCTTGAAAAAGACCGCTTAACCGAGCATATGGATGATGCATTTGAGGTGCAGAGCCGGCTGGA
TCGAAAAGATGTATACTATGATCAGCACGGGAAGCTGGCACTCATCCCTTTTACAGTCCAGACACGCAGCTACGTCATCA
AGCTGGCGTCAGACAGCATTGTAACAGAATTTGATTATTTGCTTTTCACATCATTAACTAGCATTTACGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGACGGATAA
Antitoxin
Download Length: 57 a.a. Molecular weight: 6639.60 Da Isoelectric Point: 11.0258
>AT61835 WP_013390483.1 NZ_CP014840:2675971-2676141 [Bacillus subtilis subsp. globigii]
MERAFQNDHSTRTAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA
MERAFQNDHSTRTAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA
Download Length: 171 bp
>AT61835 NZ_CP014840:2675971-2676141 [Bacillus subtilis subsp. globigii]
ATGGAACGTGCTTTTCAAAACGATCATTCGACCCGCACGGCTAAACCGTTTAAGATATTAAAAAAGAGATCAACCACCAG
CGTCGCAAGCTATCAGGTCAGCCCCCACACGGCAAGAATTTTCAAAGAAAATGAGCGGCTGATTGACGAGTATAAAAGAA
AAAAAGCATGA
ATGGAACGTGCTTTTCAAAACGATCATTCGACCCGCACGGCTAAACCGTTTAAGATATTAAAAAAGAGATCAACCACCAG
CGTCGCAAGCTATCAGGTCAGCCCCCACACGGCAAGAATTTTCAAAGAAAATGAGCGGCTGATTGACGAGTATAAAAGAA
AAAAAGCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U5BWH7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U5D589 |