Detailed information of TA system TA061827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 489837..490474
• Replicon: chromosome
• Accession: NZ_CP014838
• Organism: Bacillus velezensis strain CBMB205

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BCBMB205_RS02535
• Protein ID: WP_003156187.1
• Coordinates: 490124..490474 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: BCBMB205_RS02530
• Protein ID: WP_003156188.1
• Coordinates: 489837..490118 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCBMB205_RS02510	486202..486801	-	600	WP_032872997.1	rhomboid family intramembrane serine protease	-
BCBMB205_RS02515	486894..487259	+	366	WP_007609580.1	holo-ACP synthase	-
BCBMB205_RS02520	487424..488431	+	1008	WP_032872999.1	outer membrane lipoprotein carrier protein LolA	-
BCBMB205_RS02525	488548..489717	+	1170	WP_032873001.1	alanine racemase	-
BCBMB205_RS02530	489837..490118	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BCBMB205_RS02535	490124..490474	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BCBMB205_RS02540	490592..491413	+	822	WP_003156182.1	STAS domain-containing protein	-
BCBMB205_RS02545	491418..491783	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
BCBMB205_RS02550	491786..492187	+	402	WP_003156178.1	anti-sigma regulatory factor	-
BCBMB205_RS02555	492199..493206	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
BCBMB205_RS02560	493270..493599	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
BCBMB205_RS02565	493596..494078	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
BCBMB205_RS02570	494044..494832	+	789	WP_032873003.1	RNA polymerase sigma factor SigB	-
BCBMB205_RS02575	494832..495434	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T61827 WP_003156187.1 NZ_CP014838:490124-490474 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T61827 NZ_CP014838:490124-490474 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT61827 WP_003156188.1 NZ_CP014838:489837-490118 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT61827 NZ_CP014838:489837-490118 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5