Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-mazE/MazF(toxin)
Location 2528778..2529381 Replicon chromosome
Accession NZ_CP014806
Organism Rummeliibacillus stabekisii strain PP9

Toxin (Protein)


Gene name pemK Uniprot ID A0A143HEK3
Locus tag ATY39_RS12570 Protein ID WP_066790273.1
Coordinates 2528778..2529128 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A143HH95
Locus tag ATY39_RS12575 Protein ID WP_066791971.1
Coordinates 2529133..2529381 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ATY39_RS12545 2524064..2526217 - 2154 WP_066790263.1 RNA-binding transcriptional accessory protein -
ATY39_RS12550 2526321..2527100 - 780 WP_066790265.1 RNA polymerase sigma factor SigB -
ATY39_RS12555 2527078..2527548 - 471 WP_066790267.1 anti-sigma B factor RsbW -
ATY39_RS12560 2527535..2527876 - 342 WP_066790269.1 STAS domain-containing protein -
ATY39_RS12565 2528066..2528479 - 414 WP_066790271.1 anti-sigma regulatory factor -
ATY39_RS12570 2528778..2529128 - 351 WP_066790273.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ATY39_RS12575 2529133..2529381 - 249 WP_066791971.1 hypothetical protein Antitoxin
ATY39_RS12580 2529623..2530747 - 1125 WP_066790275.1 alanine racemase -
ATY39_RS12585 2530922..2532211 - 1290 WP_066790280.1 hypothetical protein -
ATY39_RS12590 2532247..2532600 - 354 WP_066790282.1 holo-ACP synthase -
ATY39_RS12595 2532669..2533277 + 609 WP_066790283.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12818.94 Da        Isoelectric Point: 7.0151

>T61826 WP_066790273.1 NZ_CP014806:c2529128-2528778 [Rummeliibacillus stabekisii]
MNVKRGDVFFADLSPVVGSEQGGTRPVLVIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQL
RTIDKSRLTDKITQLDGPLMVKVEEALEISLGLLKF

Download         Length: 351 bp

>T61826 NZ_CP014806:c2529128-2528778 [Rummeliibacillus stabekisii]
TTGAACGTAAAACGTGGAGACGTTTTTTTTGCAGATTTATCACCAGTAGTCGGGTCTGAGCAAGGTGGCACCAGACCCGT
ACTGGTTATTCAAAATGATATCGGAAATCGGTTTAGTCCAACTGTTATTATCGCAGCAATTACAGCTCAAATTCAAAAGG
CGAAACTCCCAACTCATGTGGAAATTGACGCTAAGAAATATGGATTTGAACGGGATTCAGTTATTTTGTTGGAACAATTG
CGGACGATCGATAAATCACGATTAACAGATAAAATCACGCAGCTAGATGGCCCTTTAATGGTAAAGGTAGAAGAAGCTTT
GGAAATAAGTTTAGGGCTTTTAAAATTTTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9491.93 Da        Isoelectric Point: 6.4750

>AT61826 WP_066791971.1 NZ_CP014806:c2529381-2529133 [Rummeliibacillus stabekisii]
MPERLVSESIEQAYRQIVLKDANDLVYIPTKRSIHTKQKNHMREAMVKGYVEMSQINLSICSECLHVEYEAEHMVERLVS
GG

Download         Length: 249 bp

>AT61826 NZ_CP014806:c2529381-2529133 [Rummeliibacillus stabekisii]
ATGCCAGAACGATTGGTTTCTGAAAGTATAGAACAAGCATATAGACAAATAGTATTAAAAGACGCAAATGATTTAGTATA
TATTCCGACAAAAAGATCTATTCATACGAAACAAAAAAATCACATGCGAGAAGCAATGGTTAAAGGATATGTAGAAATGT
CACAGATTAACTTATCTATATGTAGTGAATGCTTACATGTTGAGTATGAAGCGGAACATATGGTGGAGCGACTCGTAAGC
GGAGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A143HEK3

Antitoxin

Source ID Structure
AlphaFold DB A0A143HH95

References