Detailed information of TA system TA061819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3745326..3746245
• Replicon: chromosome
• Accession: NZ_CP014795
• Organism: Bacillus licheniformis strain SCK B11

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: T5HIF3
• Locus tag: BaDB11_RS19485
• Protein ID: WP_003180879.1
• Coordinates: 3745499..3746245 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: Q65L00
• Locus tag: BaDB11_RS19480
• Protein ID: WP_011197812.1
• Coordinates: 3745326..3745499 (-)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BaDB11_RS19460	3740751..3741848	+	1098	WP_017474398.1	mannonate dehydratase	-
BaDB11_RS19465	3741824..3742672	+	849	WP_009328728.1	SDR family oxidoreductase	-
BaDB11_RS19470	3742699..3743712	+	1014	WP_017474397.1	zinc-binding alcohol dehydrogenase family protein	-
BaDB11_RS19475	3743765..3745036	+	1272	WP_011197811.1	MFS transporter	-
BaDB11_RS19480	3745326..3745499	-	174	WP_011197812.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
BaDB11_RS19485	3745499..3746245	-	747	WP_003180879.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BaDB11_RS19490	3746356..3747354	-	999	WP_009328723.1	inorganic phosphate transporter	-
BaDB11_RS19495	3747367..3747984	-	618	WP_003180884.1	DUF47 domain-containing protein	-
BaDB11_RS19500	3748317..3750074	+	1758	WP_017474396.1	gamma-glutamyltransferase	-
BaDB11_RS19505	3750211..3750855	+	645	WP_080618615.1	YesL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-57)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29391.80 Da
• Isoelectric Point: 4.5367

>T61819 WP_003180879.1 NZ_CP014795:c3746245-3745499 [Bacillus licheniformis]
MLLFYQFLVWLIVLALALYVAAVWRFEKQLAEKTVAIRKTWYLLYVIGAVIYWTHDPQSIFTNPLHYLIVAVFFTLTDAF
IFLNAYFKKLGSSELATDTRMLLEENNDLLHTYQNRLKTFQYLLKNEPIHIYYGNIEAYAEGIEKLIKRFAEKMNISAAL
CEYNSEESKDHLLEHMENRFDVQEKLDRKDVYYEENGKMVLIPFSIHDFDYVMKLTSEDLVTEFDYLLFTSLTSIYDLLL
PNEEEGDD

Nucleotide

• Length: 747 bp

>T61819 NZ_CP014795:c3746245-3745499 [Bacillus licheniformis]
ATGCTGCTATTTTATCAATTCCTTGTTTGGCTTATTGTTTTGGCGCTGGCTCTGTACGTAGCTGCCGTGTGGCGTTTTGA
AAAACAGCTCGCCGAAAAAACGGTCGCCATCAGAAAAACGTGGTACCTTCTTTATGTCATCGGCGCCGTGATATATTGGA
CGCACGATCCGCAGTCAATTTTCACAAACCCGCTTCATTACCTGATCGTTGCCGTTTTTTTCACGTTGACAGACGCTTTT
ATTTTCTTAAATGCCTACTTTAAAAAGCTCGGCAGCTCTGAACTCGCGACAGATACAAGAATGCTTCTCGAAGAAAACAA
CGACCTCCTCCACACGTATCAAAACAGGCTGAAAACGTTTCAATACCTATTGAAAAACGAACCGATCCACATCTATTATG
GAAATATAGAAGCGTATGCAGAAGGCATCGAAAAGCTCATCAAACGGTTTGCCGAAAAAATGAATATATCCGCTGCACTT
TGCGAATATAATTCAGAAGAAAGCAAGGATCATTTGCTTGAGCATATGGAAAACCGGTTCGATGTTCAAGAAAAGCTTGA
CCGCAAAGACGTTTATTATGAAGAAAACGGAAAAATGGTCCTCATTCCTTTTTCCATCCACGATTTTGACTATGTCATGA
AGTTAACCTCAGAAGACCTTGTCACAGAATTTGATTATCTCCTGTTTACTTCTTTAACAAGCATCTATGATCTTCTGCTA
CCAAACGAAGAGGAAGGTGACGACTGA

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6971.08 Da
• Isoelectric Point: 10.7815

>AT61819 WP_011197812.1 NZ_CP014795:c3745499-3745326 [Bacillus licheniformis]
MEPAFQQDQESVKKRLAFKMFKRQSKTNIAKYEVSPYTERIFRQNERLIGEYKRKKA

Nucleotide

• Length: 174 bp

>AT61819 NZ_CP014795:c3745499-3745326 [Bacillus licheniformis]
ATGGAGCCTGCTTTTCAGCAAGACCAGGAAAGCGTAAAAAAGCGCCTCGCCTTTAAAATGTTTAAAAGGCAATCCAAAAC
CAATATCGCGAAATACGAAGTCAGCCCCTATACCGAACGGATTTTCCGGCAAAACGAACGGCTGATCGGCGAGTATAAAA
GAAAAAAAGCTTAA

Structures

Toxin

• AlphaFold DB: T5HIF3

Antitoxin

• AlphaFold DB: Q65L00