Detailed information of TA system TA061818

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2953345..2953981
• Replicon: chromosome
• Accession: NZ_CP014795
• Organism: Bacillus licheniformis strain SCK B11

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: M5P3Q9
• Locus tag: BaDB11_RS15390
• Protein ID: WP_003179128.1
• Coordinates: 2953631..2953981 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M5PDU2
• Locus tag: BaDB11_RS15385
• Protein ID: WP_006638778.1
• Coordinates: 2953345..2953626 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BaDB11_RS15365	2948460..2949941	+	1482	WP_009330132.1	PH domain-containing protein	-
BaDB11_RS15370	2949938..2950537	-	600	WP_003179118.1	rhomboid family intramembrane serine protease	-
BaDB11_RS15375	2950880..2951839	+	960	WP_152847888.1	outer membrane lipoprotein carrier protein LolA	-
BaDB11_RS15380	2952064..2953233	+	1170	WP_025807384.1	alanine racemase	-
BaDB11_RS15385	2953345..2953626	+	282	WP_006638778.1	hypothetical protein	Antitoxin
BaDB11_RS15390	2953631..2953981	+	351	WP_003179128.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BaDB11_RS15395	2954099..2954926	+	828	WP_003179130.1	RsbT co-antagonist protein RsbRA	-
BaDB11_RS15400	2954930..2955295	+	366	WP_003179132.1	STAS domain-containing protein	-
BaDB11_RS15405	2955298..2955699	+	402	WP_003179135.1	anti-sigma regulatory factor	-
BaDB11_RS15410	2955710..2956717	+	1008	WP_003179137.1	PP2C family protein-serine/threonine phosphatase	-
BaDB11_RS15415	2956776..2957102	+	327	WP_003179140.1	anti-sigma factor antagonist	-
BaDB11_RS15420	2957102..2957587	+	486	WP_003179142.1	anti-sigma B factor RsbW	-
BaDB11_RS15425	2957553..2958344	+	792	WP_003179144.1	RNA polymerase sigma factor SigB	-
BaDB11_RS15430	2958341..2958940	+	600	WP_003179145.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12990.03 Da
• Isoelectric Point: 5.7234

>T61818 WP_003179128.1 NZ_CP014795:2953631-2953981 [Bacillus licheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Nucleotide

• Length: 351 bp

>T61818 NZ_CP014795:2953631-2953981 [Bacillus licheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGACTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10649.03 Da
• Isoelectric Point: 4.6920

>AT61818 WP_006638778.1 NZ_CP014795:2953345-2953626 [Bacillus licheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Nucleotide

• Length: 282 bp

>AT61818 NZ_CP014795:2953345-2953626 [Bacillus licheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTACGCGAACGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATCAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A6I7FHI4

Antitoxin

• AlphaFold DB: M5PDU2