Detailed information of TA system TA061815

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 4244018..4244937
• Replicon: chromosome
• Accession: NZ_CP014793
• Organism: Bacillus licheniformis strain SCDB 34

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: T5HIF3
• Locus tag: B34_RS22175
• Protein ID: WP_003180879.1
• Coordinates: 4244191..4244937 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: T5HT37
• Locus tag: B34_RS22170
• Protein ID: WP_003180877.1
• Coordinates: 4244018..4244191 (-)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B34_RS22150	4239443..4240540	+	1098	WP_003180867.1	mannonate dehydratase	-
B34_RS22155	4240516..4241364	+	849	WP_003180869.1	SDR family oxidoreductase	-
B34_RS22160	4241391..4242404	+	1014	WP_011197810.1	zinc-binding alcohol dehydrogenase family protein	-
B34_RS22165	4242457..4243728	+	1272	WP_009328725.1	MFS transporter	-
B34_RS22170	4244018..4244191	-	174	WP_003180877.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
B34_RS22175	4244191..4244937	-	747	WP_003180879.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
B34_RS22180	4245048..4246046	-	999	WP_009328723.1	inorganic phosphate transporter	-
B34_RS22185	4246059..4246676	-	618	WP_003180884.1	DUF47 domain-containing protein	-
B34_RS22190	4247008..4248765	+	1758	WP_011197813.1	gamma-glutamyltransferase	-
B34_RS22195	4248894..4249537	+	644	Protein_4306	DUF624 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-57)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29391.80 Da
• Isoelectric Point: 4.5367

>T61815 WP_003180879.1 NZ_CP014793:c4244937-4244191 [Bacillus licheniformis]
MLLFYQFLVWLIVLALALYVAAVWRFEKQLAEKTVAIRKTWYLLYVIGAVIYWTHDPQSIFTNPLHYLIVAVFFTLTDAF
IFLNAYFKKLGSSELATDTRMLLEENNDLLHTYQNRLKTFQYLLKNEPIHIYYGNIEAYAEGIEKLIKRFAEKMNISAAL
CEYNSEESKDHLLEHMENRFDVQEKLDRKDVYYEENGKMVLIPFSIHDFDYVMKLTSEDLVTEFDYLLFTSLTSIYDLLL
PNEEEGDD

Nucleotide

• Length: 747 bp

>T61815 NZ_CP014793:c4244937-4244191 [Bacillus licheniformis]
ATGCTGCTATTTTATCAATTCCTTGTTTGGCTTATTGTTTTGGCGCTGGCTCTGTACGTAGCTGCCGTGTGGCGTTTTGA
AAAACAGCTCGCCGAAAAAACGGTCGCCATCAGAAAAACGTGGTACCTTCTTTATGTCATCGGCGCCGTGATATATTGGA
CGCACGATCCGCAGTCAATTTTCACAAACCCGCTTCATTACCTGATCGTTGCCGTTTTTTTCACGTTGACAGACGCTTTT
ATTTTCTTAAATGCCTACTTTAAAAAGCTCGGCAGCTCTGAACTCGCGACAGATACAAGAATGCTTCTCGAAGAAAACAA
CGACCTCCTCCACACGTATCAAAACAGGCTGAAAACGTTTCAATACCTATTGAAAAACGAACCGATCCACATCTATTATG
GAAATATAGAAGCGTATGCAGAAGGCATCGAAAAGCTCATCAAACGGTTTGCCGAAAAAATGAATATATCCGCTGCACTT
TGCGAATATAATTCAGAAGAAAGCAAGGATCATTTGCTTGAGCATATGGAAAACCGGTTCGATGTTCAAGAAAAGCTTGA
CCGCAAAGACGTTTATTATGAAGAAAACGGAAAAATGGTCCTCATTCCTTTTTCCATCCACGATTTTGACTATGTCATGA
AGTTAACCTCAGAAGACCTTGTCACAGAATTTGATTATCTCCTGTTTACTTCTTTAACAAGCATCTATGATCTTCTGCTA
CCAAACGAAGAGGAAGGTGACGACTGA

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6945.04 Da
• Isoelectric Point: 10.9186

>AT61815 WP_003180877.1 NZ_CP014793:c4244191-4244018 [Bacillus licheniformis]
MEPAFQQDQESVKKRLAFKMFKRQSKTNIAKYEVSPHTERIFRQNERLIGEYKRKKA

Nucleotide

• Length: 174 bp

>AT61815 NZ_CP014793:c4244191-4244018 [Bacillus licheniformis]
ATGGAGCCTGCTTTTCAGCAAGACCAGGAAAGCGTAAAAAAGCGCCTCGCCTTTAAAATGTTTAAAAGGCAATCCAAAAC
CAATATCGCGAAATACGAAGTCAGCCCCCATACCGAACGGATTTTCCGGCAAAACGAACGGCTGATCGGCGAGTATAAAA
GAAAAAAAGCTTAA

Structures

Toxin

• AlphaFold DB: T5HIF3

Antitoxin

• AlphaFold DB: T5HT37