Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3381879..3382515 Replicon chromosome
Accession NZ_CP014793
Organism Bacillus licheniformis strain SCDB 34

Toxin (Protein)


Gene name mazF Uniprot ID M5P3Q9
Locus tag B34_RS17655 Protein ID WP_003179128.1
Coordinates 3382165..3382515 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M5PDU2
Locus tag B34_RS17650 Protein ID WP_006638778.1
Coordinates 3381879..3382160 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B34_RS17630 3376994..3378475 + 1482 WP_009330132.1 PH domain-containing protein -
B34_RS17635 3378472..3379071 - 600 WP_003179118.1 rhomboid family intramembrane serine protease -
B34_RS17640 3379414..3380373 + 960 WP_152847888.1 outer membrane lipoprotein carrier protein LolA -
B34_RS17645 3380598..3381767 + 1170 WP_026080763.1 alanine racemase -
B34_RS17650 3381879..3382160 + 282 WP_006638778.1 hypothetical protein Antitoxin
B34_RS17655 3382165..3382515 + 351 WP_003179128.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
B34_RS17660 3382633..3383460 + 828 WP_061578564.1 RsbT co-antagonist protein RsbRA -
B34_RS17665 3383464..3383829 + 366 WP_003179132.1 STAS domain-containing protein -
B34_RS17670 3383832..3384233 + 402 WP_003179135.1 anti-sigma regulatory factor -
B34_RS17675 3384244..3385251 + 1008 WP_003179137.1 PP2C family protein-serine/threonine phosphatase -
B34_RS17680 3385310..3385636 + 327 WP_003179140.1 anti-sigma factor antagonist -
B34_RS17685 3385636..3386121 + 486 WP_003179142.1 anti-sigma B factor RsbW -
B34_RS17690 3386087..3386878 + 792 WP_003179144.1 RNA polymerase sigma factor SigB -
B34_RS17695 3386875..3387474 + 600 WP_003179145.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12990.03 Da        Isoelectric Point: 5.7234

>T61814 WP_003179128.1 NZ_CP014793:3382165-3382515 [Bacillus licheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Download         Length: 351 bp

>T61814 NZ_CP014793:3382165-3382515 [Bacillus licheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGACTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10649.03 Da        Isoelectric Point: 4.6920

>AT61814 WP_006638778.1 NZ_CP014793:3381879-3382160 [Bacillus licheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Download         Length: 282 bp

>AT61814 NZ_CP014793:3381879-3382160 [Bacillus licheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTACGCGAACGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATCAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I7FHI4

Antitoxin

Source ID Structure
AlphaFold DB M5PDU2

References