Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2017536..2018050 Replicon chromosome
Accession NZ_CP014791
Organism Staphylococcus aureus strain MCRF184

Toxin (Protein)


Gene name TsdT Uniprot ID A0A0U1MQX5
Locus tag CKU_RS10000 Protein ID WP_000581795.1
Coordinates 2017536..2017745 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID A0A0U1MQV0
Locus tag CKU_RS10005 Protein ID WP_000138930.1
Coordinates 2017757..2018050 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKU_RS09980 2013044..2014402 - 1359 WP_000611479.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
CKU_RS09985 2014417..2015487 - 1071 WP_000159641.1 D-alanine--D-alanine ligase -
CKU_RS09990 2015805..2017007 + 1203 WP_001109939.1 rod shape-determining protein RodA -
CKU_RS09995 2017271..2017408 - 138 WP_000828354.1 hypothetical protein -
CKU_RS10000 2017536..2017745 - 210 WP_000581795.1 heavy-metal-associated domain-containing protein Toxin
CKU_RS10005 2017757..2018050 - 294 WP_000138930.1 copper-sensing transcriptional repressor CsoR Antitoxin
CKU_RS10010 2018216..2019700 + 1485 WP_000571562.1 cardiolipin synthase -
CKU_RS10015 2019728..2020375 + 648 WP_001187625.1 HD domain-containing protein -
CKU_RS10020 2020925..2021797 - 873 WP_000725802.1 membrane protein insertase YidC -
CKU_RS10025 2021883..2022524 - 642 WP_000483162.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7842.83 Da        Isoelectric Point: 4.3869

>T61811 WP_000581795.1 NZ_CP014791:c2017745-2017536 [Staphylococcus aureus]
MIHQNTIYTAGIETKEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T61811 NZ_CP014791:c2017745-2017536 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAAAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11169.81 Da        Isoelectric Point: 6.2242

>AT61811 WP_000138930.1 NZ_CP014791:c2018050-2017757 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVKAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT61811 NZ_CP014791:c2018050-2017757 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAAAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CATTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0U1MQX5

Antitoxin

Source ID Structure
AlphaFold DB A0A0U1MQV0

References