Detailed information of TA system TA061810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1997793..1998322
• Replicon: chromosome
• Accession: NZ_CP014791
• Organism: Staphylococcus aureus strain MCRF184

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CKU_RS09910
• Protein ID: WP_000621175.1
• Coordinates: 1997793..1998155 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: CKU_RS09915
• Protein ID: WP_000948331.1
• Coordinates: 1998152..1998322 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKU_RS09885	1994772..1995542	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
CKU_RS09890	1995517..1995996	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
CKU_RS09895	1995998..1996324	-	327	WP_061869739.1	anti-sigma factor antagonist	-
CKU_RS09900	1996443..1997444	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
CKU_RS09910	1997793..1998155	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CKU_RS09915	1998152..1998322	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CKU_RS09920	1998407..1999555	-	1149	WP_001281140.1	alanine racemase	-
CKU_RS09925	1999621..1999980	-	360	WP_000581199.1	holo-ACP synthase	-
CKU_RS09930	1999984..2000475	-	492	WP_001205907.1	PH domain-containing protein	-
CKU_RS09935	2000462..2002045	-	1584	WP_001294637.1	PH domain-containing protein	-
CKU_RS09940	2002038..2002517	-	480	WP_001287077.1	hypothetical protein	-
CKU_RS09945	2002725..2003285	-	561	WP_001092406.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T61810 WP_000621175.1 NZ_CP014791:c1998155-1997793 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T61810 NZ_CP014791:c1998155-1997793 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT61810 WP_000948331.1 NZ_CP014791:c1998322-1998152 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT61810 NZ_CP014791:c1998322-1998152 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1