Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /Xre(antitoxin)
Location 3510806..3512022 Replicon chromosome
Accession NZ_CP014784
Organism Pseudomonas alcaligenes strain NEB 585

Toxin (Protein)


Gene name - Uniprot ID A0A142IU31
Locus tag A0T30_RS16080 Protein ID WP_061904972.1
Coordinates 3510806..3511558 (-) Length 251 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A142IU32
Locus tag A0T30_RS16085 Protein ID WP_061904973.1
Coordinates 3511651..3512022 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A0T30_RS16060 3506602..3507015 - 414 WP_021701970.1 sel1 repeat family protein -
A0T30_RS16065 3507444..3507827 + 384 WP_061904969.1 DUF2946 family protein -
A0T30_RS16070 3507924..3510065 + 2142 WP_061904970.1 TonB-dependent receptor -
A0T30_RS16075 3510341..3510805 + 465 WP_061904971.1 copper chaperone PCu(A)C -
A0T30_RS16080 3510806..3511558 - 753 WP_061904972.1 RES family NAD+ phosphorylase Toxin
A0T30_RS16085 3511651..3512022 - 372 WP_061904973.1 DUF2384 domain-containing protein Antitoxin
A0T30_RS16090 3512336..3513559 - 1224 WP_061904974.1 organoarsenical effux MFS transporter ArsJ -
A0T30_RS16095 3513569..3514573 - 1005 WP_061904975.1 ArsJ-associated glyceraldehyde-3-phosphate dehydrogenase -
A0T30_RS16100 3514630..3515412 - 783 WP_061904976.1 metalloregulator ArsR/SmtB family transcription factor -
A0T30_RS16105 3515518..3516597 + 1080 WP_061904977.1 ACR3 family arsenite efflux transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(64-206)

Antitoxin

Antitoxin Xre/MbcA/ParS-like, toxin-binding domain

(72-121)


Sequences

Toxin        


Download         Length: 251 a.a.        Molecular weight: 28266.24 Da        Isoelectric Point: 7.0306

>T61805 WP_061904972.1 NZ_CP014784:c3511558-3510806 [Pseudomonas alcaligenes]
MDIWQACAGVEQIIPLRGRLVRLVENQGQVATLQLVDTLEEQALLEQLLESSKPPLPPGDEPLHYLLRTPFRYPPLRWGS
RFGRRHEPSLFYAACRLETAMAETAFYRFVLWYGMVEPPPSGRILSEHSSFEARYQVQRGVRLQLPPFDGHRALLTHRSD
YRATHSLGSAMREAGVEAFEYPSARCPQGGNNVALYAPTAFSEKRPRNLQPWLCETTAGYVAFKSAQAPDTPRLFALELY
LEDGVLPQPA

Download         Length: 753 bp

>T61805 NZ_CP014784:c3511558-3510806 [Pseudomonas alcaligenes]
ATGGATATCTGGCAGGCCTGTGCCGGCGTAGAGCAGATCATTCCCCTGCGCGGCCGCCTGGTGCGCCTGGTCGAGAACCA
GGGCCAGGTGGCAACCCTGCAGCTGGTGGATACGCTGGAGGAGCAGGCGCTACTCGAACAATTGCTGGAGAGCAGCAAGC
CGCCGCTGCCTCCCGGTGACGAGCCGTTGCACTACCTGTTGCGCACGCCGTTTCGCTACCCGCCGCTGCGCTGGGGCTCG
CGTTTCGGCAGGCGCCATGAGCCGAGCCTGTTCTACGCCGCCTGCCGGCTGGAGACGGCGATGGCCGAGACGGCCTTCTA
TCGTTTCGTGCTGTGGTACGGCATGGTCGAGCCTCCGCCGAGCGGACGCATCCTTTCCGAGCATTCGTCCTTCGAGGCGC
GCTACCAGGTACAGCGCGGGGTACGCCTGCAGCTGCCGCCATTCGACGGCCATCGCGCGCTGCTGACCCATCGCAGCGAC
TATCGCGCCACCCACAGTCTGGGCAGCGCGATGCGCGAAGCTGGGGTGGAAGCGTTCGAGTATCCCTCGGCACGCTGCCC
GCAGGGGGGCAACAACGTTGCCCTGTATGCGCCGACGGCGTTCAGCGAGAAGCGGCCGCGCAACCTGCAGCCCTGGCTAT
GCGAAACCACGGCAGGCTACGTGGCCTTCAAGAGCGCCCAGGCGCCCGATACACCGCGCCTGTTTGCCCTCGAACTCTAT
CTCGAGGATGGCGTGCTGCCGCAACCAGCCTGA

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13364.54 Da        Isoelectric Point: 10.8344

>AT61805 WP_061904973.1 NZ_CP014784:c3512022-3511651 [Pseudomonas alcaligenes]
MSALSKPGVDAGAVLLKALLNSREQLGLTQQELAAIVGVNRSAISRWSDSGGLRPESKTGELALLLIRAYRALFALFGGN
LQDMRHFLRTENRHLAGVPLQQMGQVQGLVRVVEYLDAIRGKV

Download         Length: 372 bp

>AT61805 NZ_CP014784:c3512022-3511651 [Pseudomonas alcaligenes]
ATGAGCGCACTCAGCAAGCCAGGTGTCGATGCCGGCGCCGTCCTGCTCAAGGCCCTGCTCAACAGCCGTGAGCAGCTGGG
CCTGACCCAGCAGGAGCTGGCGGCGATAGTCGGCGTCAACCGCTCCGCCATCAGTCGCTGGAGCGACAGCGGCGGCCTGC
GTCCGGAGAGCAAGACCGGTGAGCTGGCCTTGCTGCTGATCCGCGCCTATCGCGCCCTGTTCGCCCTGTTCGGCGGCAAT
CTGCAGGACATGCGGCATTTCCTGCGCACCGAGAATCGCCATCTGGCCGGCGTGCCCTTGCAGCAGATGGGGCAGGTACA
GGGGCTGGTGCGGGTGGTCGAGTACCTGGACGCCATTCGCGGCAAGGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A142IU31

Antitoxin

Source ID Structure
AlphaFold DB A0A142IU32

References