Detailed information of TA system TA061802

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 495056..495693
• Replicon: chromosome
• Accession: NZ_CP014783
• Organism: Bacillus amyloliquefaciens strain B15

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: A1R12_RS02490
• Protein ID: WP_003156187.1
• Coordinates: 495343..495693 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: A1R12_RS02485
• Protein ID: WP_003156188.1
• Coordinates: 495056..495337 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A1R12_RS02465	491421..492020	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
A1R12_RS02470	492113..492478	+	366	WP_007410229.1	holo-ACP synthase	-
A1R12_RS02475	492643..493650	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
A1R12_RS02480	493767..494936	+	1170	WP_061890435.1	alanine racemase	-
A1R12_RS02485	495056..495337	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
A1R12_RS02490	495343..495693	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
A1R12_RS02495	495811..496632	+	822	WP_015416874.1	STAS domain-containing protein	-
A1R12_RS02500	496637..497002	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
A1R12_RS02505	497005..497406	+	402	WP_003156178.1	anti-sigma regulatory factor	-
A1R12_RS02510	497418..498425	+	1008	WP_061890436.1	PP2C family protein-serine/threonine phosphatase	-
A1R12_RS02515	498489..498818	+	330	WP_033575044.1	anti-sigma factor antagonist	-
A1R12_RS02520	498815..499297	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
A1R12_RS02525	499263..500051	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
A1R12_RS02530	500051..500653	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T61802 WP_003156187.1 NZ_CP014783:495343-495693 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T61802 NZ_CP014783:495343-495693 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT61802 WP_003156188.1 NZ_CP014783:495056-495337 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT61802 NZ_CP014783:495056-495337 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5