Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 273123..273859 Replicon plasmid pKPN-332
Accession NZ_CP014763
Organism Klebsiella pneumoniae strain KPNIH39

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag WM47_RS28135 Protein ID WP_004098919.1
Coordinates 273377..273859 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A2J5Q9C0
Locus tag WM47_RS28130 Protein ID WP_004098921.1
Coordinates 273123..273389 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WM47_RS28095 268695..269696 + 1002 WP_032635019.1 Abi family protein -
WM47_RS28100 269835..270413 - 579 WP_064798526.1 hypothetical protein -
WM47_RS28105 270703..270951 + 249 WP_064798517.1 hypothetical protein -
WM47_RS28110 270948..271520 + 573 WP_064798518.1 hypothetical protein -
WM47_RS28115 271551..272045 + 495 WP_064798519.1 hypothetical protein -
WM47_RS28120 272235..272585 - 351 WP_064798520.1 hypothetical protein -
WM47_RS28125 272586..272849 - 264 WP_064798521.1 hypothetical protein -
WM47_RS28130 273123..273389 + 267 WP_004098921.1 DUF1778 domain-containing protein Antitoxin
WM47_RS28135 273377..273859 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
WM47_RS28140 274018..275550 - 1533 WP_040215144.1 IS3-like element ISKpn38 family transposase -
WM47_RS28145 275670..277016 + 1347 WP_077256184.1 ISNCY family transposase -
WM47_RS28150 277075..277878 + 804 WP_040216539.1 hypothetical protein -
WM47_RS30535 277902..278572 - 671 Protein_279 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid formA mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA / pla 1..284894 284894


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T61791 WP_004098919.1 NZ_CP014763:273377-273859 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T61791 NZ_CP014763:273377-273859 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10105.49 Da        Isoelectric Point: 4.5141

>AT61791 WP_004098921.1 NZ_CP014763:273123-273389 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEIACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT61791 NZ_CP014763:273123-273389 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTAGAGATTGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A2J5Q9C0

References