Detailed information of TA system TA061771

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 654899..655569
• Replicon: chromosome
• Accession: NZ_CP014762
• Organism: Klebsiella pneumoniae strain KPNIH39

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A483GLH7
• Locus tag: WM47_RS03275
• Protein ID: WP_023301398.1
• Coordinates: 655237..655569 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A483GII7
• Locus tag: WM47_RS03270
• Protein ID: WP_023301397.1
• Coordinates: 654899..655216 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM47_RS03225	650040..650864	+	825	WP_023301389.1	DUF945 domain-containing protein	-
WM47_RS03230	651073..651783	+	711	WP_064798309.1	DeoR family transcriptional regulator	-
WM47_RS03235	651809..652345	+	537	WP_023301391.1	DUF4339 domain-containing protein	-
WM47_RS03240	652387..652824	+	438	WP_023301392.1	hypothetical protein	-
WM47_RS03245	652891..653301	+	411	WP_023301393.1	hypothetical protein	-
WM47_RS03250	653379..653615	+	237	WP_032446575.1	DUF905 domain-containing protein	-
WM47_RS03255	653701..654159	+	459	WP_023301394.1	antirestriction protein	-
WM47_RS03260	654168..654650	+	483	WP_023301395.1	RadC family protein	-
WM47_RS03265	654659..654880	+	222	WP_023301396.1	DUF987 domain-containing protein	-
WM47_RS03270	654899..655216	+	318	WP_023301397.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
WM47_RS03275	655237..655569	+	333	WP_023301398.1	TA system toxin CbtA family protein	Toxin
WM47_RS29160	656003..656863	+	861	WP_023301399.1	hypothetical protein	-
WM47_RS30650	657262..658373	+	1112	Protein_598	IS3 family transposase	-
WM47_RS31010	658390..658578	+	189	Protein_599	transposase	-
WM47_RS29175	658777..659661	-	885	WP_004192285.1	RES domain-containing protein	-
WM47_RS03295	659672..660344	-	673	Protein_601	DUF4400 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	627442..667175	39733

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-101)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12610.65 Da
• Isoelectric Point: 5.6692

>T61771 WP_023301398.1 NZ_CP014762:655237-655569 [Klebsiella pneumoniae]
MKTLPATTPQTAKLCLTSADTWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGIPLADAVNFLVDKYALVRIDRRGL
SWQEQSSYLRLVDTQRTIKVIELWLFGVMM

Nucleotide

• Length: 333 bp

>T61771 NZ_CP014762:655237-655569 [Klebsiella pneumoniae]
ATGAAAACTTTACCTGCAACAACTCCGCAGACGGCGAAGCTATGTCTGACGTCCGCGGATACCTGGCAAATGTTACTGAC
ACGTCTGCTAGAACAGCATTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATTG
ATGCAGGGATCCCTCTGGCCGATGCAGTTAACTTTCTGGTGGATAAGTACGCACTGGTTCGTATCGATCGCAGAGGATTG
AGTTGGCAGGAGCAATCATCCTATCTCCGGCTTGTCGATACTCAGCGAACCATTAAGGTTATTGAGTTGTGGCTATTTGG
TGTAATGATGTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11785.37 Da
• Isoelectric Point: 6.6277

>AT61771 WP_023301397.1 NZ_CP014762:654899-655216 [Klebsiella pneumoniae]
MSNITWGLQRDITPCLGSRLVQEGNRLHYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCVTLYH
NGFTCEADTLGSSGYVYIAVYLTQR

Nucleotide

• Length: 318 bp

>AT61771 NZ_CP014762:654899-655216 [Klebsiella pneumoniae]
ATGAGCAACATCACTTGGGGCCTGCAGCGGGATATCACGCCGTGCCTGGGATCACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTATCTGGCTGACCGAGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGTTGGATGTCGTATTTCCAC
ATTTTATCAGTCAGATGGAATCGATGTTGACCACTGGTGAAATGAACCCTCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTCCGGCTACGTATACATCGCTGTTTATCTCACCCAACGCTAA

Structures

Toxin

• AlphaFold DB: A0A483GLH7

Antitoxin

• AlphaFold DB: A0A483GII7