Detailed information of TA system TA061757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3819016..3819752
• Replicon: chromosome
• Accession: NZ_CP014755
• Organism: Klebsiella pneumoniae strain AATZP

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A4S4Y1H2
• Locus tag: WM86_RS18605
• Protein ID: WP_032433360.1
• Coordinates: 3819016..3819498 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: WM86_RS18610
• Protein ID: WP_003026799.1
• Coordinates: 3819486..3819752 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM86_RS18580	3814026..3814604	+	579	WP_032432061.1	type 1 fimbrial protein	-
WM86_RS18585	3814616..3815560	+	945	WP_077254249.1	fimbrial protein	-
WM86_RS18590	3815773..3816372	+	600	WP_064472304.1	helix-turn-helix transcriptional regulator	-
WM86_RS18595	3816752..3817384	+	633	WP_001567369.1	hypothetical protein	-
WM86_RS18600	3817413..3818816	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
WM86_RS18605	3819016..3819498	-	483	WP_032433360.1	GNAT family N-acetyltransferase	Toxin
WM86_RS18610	3819486..3819752	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
WM86_RS18615	3820065..3820628	-	564	WP_032433361.1	6-phospho-3-hexuloisomerase	-
WM86_RS18620	3820638..3821270	-	633	WP_032433362.1	3-hexulose-6-phosphate synthase	-
WM86_RS18625	3821288..3822292	-	1005	WP_032433364.1	dihydroxyacetone kinase subunit DhaK	-
WM86_RS18630	3822276..3822938	-	663	WP_032433366.1	dihydroxyacetone kinase subunit L	-
WM86_RS18635	3822967..3824106	-	1140	WP_032433368.1	mannitol dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3806963..3851807	44844
	-	flank	IS/Tn	-	-	3817413..3818816	1403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17421.02 Da
• Isoelectric Point: 9.6047

>T61757 WP_032433360.1 NZ_CP014755:c3819498-3819016 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T61757 NZ_CP014755:c3819498-3819016 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT61757 WP_003026799.1 NZ_CP014755:c3819752-3819486 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT61757 NZ_CP014755:c3819752-3819486 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A4S4Y1H2

Antitoxin

• AlphaFold DB: A0A1Q8YL66