Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 3374098..3374734 Replicon chromosome
Accession NZ_CP014749
Organism Geobacillus sp. JS12

Toxin (Protein)


Gene name mazF Uniprot ID L7ZVP1
Locus tag A0V43_RS16600 Protein ID WP_003253417.1
Coordinates 3374384..3374734 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0D8C733
Locus tag A0V43_RS16595 Protein ID WP_013144044.1
Coordinates 3374098..3374379 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A0V43_RS16575 3370358..3370975 - 618 WP_011229737.1 rhomboid family intramembrane serine protease -
A0V43_RS16580 3371089..3371478 + 390 WP_063193589.1 holo-ACP synthase -
A0V43_RS16585 3371558..3372571 + 1014 WP_063193590.1 outer membrane lipoprotein carrier protein LolA -
A0V43_RS16590 3372820..3373974 + 1155 WP_063193591.1 alanine racemase -
A0V43_RS16595 3374098..3374379 + 282 WP_013144044.1 hypothetical protein Antitoxin
A0V43_RS16600 3374384..3374734 + 351 WP_003253417.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
A0V43_RS16605 3375000..3377165 + 2166 WP_063193592.1 RNA-binding transcriptional accessory protein -
A0V43_RS19475 3377184..3377297 - 114 WP_013144046.1 cortex morphogenetic protein CmpA -
A0V43_RS16610 3377387..3377836 + 450 WP_013144047.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12992.00 Da        Isoelectric Point: 4.8781

>T61734 WP_003253417.1 NZ_CP014749:3374384-3374734 [Geobacillus sp. JS12]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLGLIDF

Download         Length: 351 bp

>T61734 NZ_CP014749:3374384-3374734 [Geobacillus sp. JS12]
TTGATTGTCAAACGTGGCGACGTGTATTTTGCGGACCTTTCCCCGGTTGTTGGCTCGGAGCAGGGCGGCGTGCGCCCCGT
GTTGGTGATCCAAAACGATATCGGCAATCGTTTTAGCCCGACGGTGATTGTCGCGGCGATTACGGCGCAAATCCAAAAAG
CGAAGCTGCCGACGCATGTCGAGATTGACGCGAAACGCTACGGGTTTGAACGCGATTCGGTCATTTTGCTTGAGCAAATT
CGCACGATCGACAAGCAACGGCTGACCGATAAAATCACTCATTTGGACGATGAAATGATGGATAAAGTCGATGAGGCGCT
GCAAATTAGCTTAGGACTGATCGACTTTTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10669.17 Da        Isoelectric Point: 7.2027

>AT61734 WP_013144044.1 NZ_CP014749:3374098-3374379 [Geobacillus sp. JS12]
VSESGATTEIVVRLPQSLLTELDVLVKQENGNRNELIYQATKMYIRERKKRQIREAMRRGYMEMAKINLSIASEAFHAEY
EADHTVERLVSGG

Download         Length: 282 bp

>AT61734 NZ_CP014749:3374098-3374379 [Geobacillus sp. JS12]
GTGTCGGAATCTGGCGCAACAACGGAAATCGTCGTTCGTTTGCCGCAGTCGCTGCTGACAGAACTGGACGTGCTCGTAAA
GCAGGAAAACGGCAACCGCAATGAACTCATTTATCAAGCGACGAAAATGTACATTCGCGAGCGGAAGAAACGGCAAATTC
GCGAGGCGATGAGACGAGGCTACATGGAAATGGCGAAAATCAATTTATCTATCGCTTCTGAAGCGTTTCATGCTGAATAC
GAGGCCGACCACACCGTTGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9ERQ4

Antitoxin

Source ID Structure
AlphaFold DB A0A0D8C733

References