Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1130583..1131181 Replicon chromosome
Accession NZ_CP014749
Organism Geobacillus sp. JS12

Toxin (Protein)


Gene name mazF Uniprot ID G8N5V4
Locus tag A0V43_RS05570 Protein ID WP_011231143.1
Coordinates 1130852..1131181 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G8N5V3
Locus tag A0V43_RS05565 Protein ID WP_011231142.1
Coordinates 1130583..1130852 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A0V43_RS05520 1125598..1125897 + 300 WP_063193816.1 transporter suffix domain-containing protein -
A0V43_RS05525 1125907..1126704 + 798 WP_013145381.1 SGNH/GDSL hydrolase family protein -
A0V43_RS05530 1126701..1127615 + 915 WP_020753915.1 ABC transporter ATP-binding protein -
A0V43_RS05535 1127608..1128588 + 981 WP_063192588.1 ABC transporter permease -
A0V43_RS05540 1128656..1129123 - 468 WP_063192589.1 hypothetical protein -
A0V43_RS05545 1129137..1129901 - 765 WP_011231140.1 hypothetical protein -
A0V43_RS05565 1130583..1130852 + 270 WP_011231142.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
A0V43_RS05570 1130852..1131181 + 330 WP_011231143.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
A0V43_RS18730 1131213..1131452 - 240 WP_020753918.1 hypothetical protein -
A0V43_RS05575 1131638..1132471 + 834 WP_015374778.1 pirin family protein -
A0V43_RS05580 1132970..1133719 + 750 WP_063192590.1 oxygen-insensitive NADPH nitroreductase -
A0V43_RS05585 1133793..1134536 + 744 WP_063192591.1 NAD-dependent protein deacylase -
A0V43_RS05590 1134717..1135894 + 1178 Protein_1150 acyl-CoA dehydrogenase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-107)

Antitoxin

Antitoxin PemI-like

(12-87)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12071.86 Da        Isoelectric Point: 7.1331

>T61733 WP_011231143.1 NZ_CP014749:1130852-1131181 [Geobacillus sp. JS12]
MQAPDRGDLVYVNFNPQAGHEQAGKRPGIVLSPKRFNQLTGFAVLCPITRQQKGYPFEVELPSGLAVEGVILTDQVKSLD
WRARQLQIVGRAPNEVVSDCLDLIHTFLS

Download         Length: 330 bp

>T61733 NZ_CP014749:1130852-1131181 [Geobacillus sp. JS12]
ATGCAAGCGCCGGATCGTGGGGATCTTGTTTACGTCAATTTCAATCCACAAGCAGGGCATGAGCAGGCAGGAAAAAGGCC
CGGCATCGTTCTATCACCAAAGCGGTTCAATCAATTGACAGGGTTTGCGGTGCTTTGCCCGATCACCCGGCAACAAAAAG
GATATCCATTTGAAGTGGAATTACCATCAGGCTTGGCCGTTGAAGGCGTGATTTTAACCGATCAGGTCAAAAGTTTAGAT
TGGCGCGCCCGACAGCTTCAAATAGTAGGACGGGCGCCCAATGAAGTTGTTTCGGATTGCTTGGATCTGATTCATACTTT
TCTTTCGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9992.67 Da        Isoelectric Point: 7.2050

>AT61733 WP_011231142.1 NZ_CP014749:1130583-1130852 [Geobacillus sp. JS12]
MELNKQKGVSIMTITVQKWGNSLAVRIPSVIAERLALHQGSEVEMIVENQAIKLIPKKKKPTLEELLAKITPENRHAEID
FGTEGNELF

Download         Length: 270 bp

>AT61733 NZ_CP014749:1130583-1130852 [Geobacillus sp. JS12]
ATGGAATTAAACAAACAGAAAGGAGTGTCAATCATGACAATAACCGTTCAAAAATGGGGAAACAGCCTTGCGGTTCGTAT
CCCAAGCGTGATTGCTGAACGTTTAGCGCTTCATCAAGGATCAGAAGTGGAGATGATCGTTGAGAACCAAGCGATCAAGT
TGATCCCGAAAAAGAAGAAGCCGACATTGGAGGAACTTTTGGCTAAAATCACGCCGGAAAATCGCCATGCTGAAATTGAT
TTTGGAACAGAAGGGAATGAATTGTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9EUB7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9HWH2

References