Detailed information of TA system TA061730

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2258304..2259325
• Replicon: chromosome
• Accession: NZ_CP014748
• Organism: Klebsiella aerogenes strain FDAARGOS_139

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: A0A0G9UXE3
• Locus tag: AL497_RS11525
• Protein ID: WP_032706547.1
• Coordinates: 2258750..2259325 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A0G9R618
• Locus tag: AL497_RS11520
• Protein ID: WP_015369110.1
• Coordinates: 2258304..2258750 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL497_RS11495	2253825..2254604	+	780	WP_015369105.1	MBL fold metallo-hydrolase	-
AL497_RS11500	2254626..2255579	+	954	WP_032706577.1	helix-turn-helix domain-containing protein	-
AL497_RS11505	2255576..2256625	-	1050	WP_015369107.1	NAD(P)-dependent alcohol dehydrogenase	-
AL497_RS11510	2256783..2257085	-	303	WP_015369108.1	antibiotic biosynthesis monooxygenase	-
AL497_RS11515	2257086..2258093	-	1008	WP_015369109.1	zinc-binding alcohol dehydrogenase family protein	-
AL497_RS11520	2258304..2258750	+	447	WP_015369110.1	helix-turn-helix domain-containing protein	Antitoxin
AL497_RS11525	2258750..2259325	+	576	WP_032706547.1	PIN domain-containing protein	Toxin
AL497_RS11535	2259630..2260826	+	1197	WP_015369112.1	hypothetical protein	-
AL497_RS11540	2261003..2262040	-	1038	WP_032706544.1	virulence RhuM family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• SinI-like, DNA-binding domain (79-126)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21688.29 Da
• Isoelectric Point: 6.2900

>T61730 WP_032706547.1 NZ_CP014748:2258750-2259325 [Klebsiella aerogenes]
MRHSPYPVILDACVLYPARLRDLLMHLGIAGLYQPKWSRFIHDEWCRNLLINRPDIAPEALRRTVDLMNAALPDANVTGF
AGLINGLVLPDPDDRHVLAAAIRAKAEIIVTLNHKDFPAESLLPFEIETLHPDVFISDLFDLNHALALEAVRRQRQSLKH
PPMTVDEFLEMLLKQGLPMTVKELANYQYAI

Nucleotide

• Length: 576 bp

>T61730 NZ_CP014748:2258750-2259325 [Klebsiella aerogenes]
ATGCGACATTCACCTTATCCGGTGATTTTAGATGCTTGTGTATTATATCCAGCACGGCTCAGGGATTTATTAATGCACTT
AGGGATCGCTGGACTTTATCAACCAAAATGGAGCCGCTTCATTCACGATGAATGGTGCAGGAATCTTCTTATAAATCGGC
CTGATATAGCACCTGAAGCGCTGCGTCGTACCGTTGATCTCATGAATGCAGCTTTACCGGATGCTAATGTAACGGGTTTT
GCAGGATTAATTAACGGACTAGTTTTACCCGACCCTGACGATCGGCATGTCCTTGCTGCGGCAATCAGAGCGAAGGCTGA
AATTATCGTTACGTTAAATCATAAAGACTTTCCAGCAGAATCATTGCTTCCATTTGAAATCGAAACACTTCATCCTGATG
TATTCATATCGGACCTGTTTGACCTTAATCATGCTCTGGCGTTGGAGGCGGTGAGAAGGCAGCGGCAGTCTCTCAAGCAT
CCTCCAATGACCGTCGATGAGTTCCTGGAGATGCTGCTAAAGCAGGGCTTGCCGATGACGGTAAAAGAGTTAGCCAATTA
CCAGTACGCCATATAG

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16699.26 Da
• Isoelectric Point: 6.2257

>AT61730 WP_015369110.1 NZ_CP014748:2258304-2258750 [Klebsiella aerogenes]
MTKLNLPDPLESELAQRGQRELAAYLSTHLETQRIAIVGEDDKTHTIELPTSALTMLMEILGELACGNAVQIVPVHAELT
TQEAANILNVSRPHMVKLLEARKLPFHKTGRHRRVLFADLMEYKKRREQESLDAMQALADQAQDLGMY

Nucleotide

• Length: 447 bp

>AT61730 NZ_CP014748:2258304-2258750 [Klebsiella aerogenes]
ATGACTAAACTGAACCTTCCGGACCCGCTGGAGAGTGAACTTGCCCAGCGCGGGCAGCGTGAGCTGGCGGCATACCTTTC
TACTCATCTTGAAACGCAGCGTATTGCGATTGTGGGTGAAGATGACAAAACTCATACCATTGAGTTGCCAACCAGCGCTC
TGACTATGCTGATGGAGATCCTTGGGGAATTGGCTTGTGGCAACGCGGTGCAGATTGTTCCTGTTCATGCTGAACTCACC
ACCCAGGAAGCAGCGAATATACTCAATGTTTCTCGTCCGCATATGGTTAAACTGCTGGAAGCGAGGAAGCTTCCATTTCA
TAAAACTGGACGCCATCGCCGGGTTCTGTTTGCCGATCTGATGGAGTATAAAAAACGTAGGGAACAGGAAAGCCTGGACG
CGATGCAAGCGCTGGCGGATCAGGCGCAGGACTTAGGGATGTATTAA

Structures

Toxin

• AlphaFold DB: A0A0G9UXE3

Antitoxin

• AlphaFold DB: A0A0G9R618