Detailed information of TA system TA061714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yxiD-yxxD/-
• Location: 3485853..3486641
• Replicon: chromosome
• Accession: NZ_CP014700
• Organism: Bacillus amyloliquefaciens strain S499

Toxin (Protein)

• Gene name: yxiD
• Uniprot ID: -
• Locus tag: AS588_RS19965
• Protein ID: WP_079891856.1
• Coordinates: 3486300..3486641 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yxxD
• Uniprot ID: -
• Locus tag: AS588_RS16785
• Protein ID: WP_015387595.1
• Coordinates: 3485853..3486290 (-)
• Length: 146 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AS588_RS16750	3481380..3481916	+	537	WP_014305969.1	chromate resistance efflux protein ChrA	-
AS588_RS16755	3481943..3482161	-	219	WP_015387600.1	hypothetical protein	-
AS588_RS16760	3482158..3482703	-	546	WP_015387599.1	flavodoxin family protein	-
AS588_RS16765	3482826..3483707	+	882	WP_015387598.1	LysR family transcriptional regulator	-
AS588_RS16770	3483794..3484510	-	717	WP_015387597.1	endonuclease V	-
AS588_RS16775	3484550..3485050	-	501	WP_015387596.1	hypothetical protein	-
AS588_RS16780	3485242..3485682	-	441	Protein_3322	SMI1/KNR4 family protein	-
AS588_RS16785	3485853..3486290	-	438	WP_015387595.1	SMI1/KNR4 family protein	Antitoxin
AS588_RS19965	3486300..3486641	-	342	WP_079891856.1	HNH endonuclease	Toxin
AS588_RS19970	3487260..3487598	-	339	Protein_3325	transposase	-
AS588_RS19645	3488237..3488702	-	466	Protein_3326	DUF2004 domain-containing protein	-
AS588_RS19760	3488704..3489229	-	526	Protein_3327	HNH endonuclease	-
AS588_RS16805	3489400..3489705	-	306	WP_003151282.1	hypothetical protein	-
AS588_RS16810	3489722..3491467	-	1746	WP_015387593.1	ribonuclease YeeF family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13224.74 Da
• Isoelectric Point: 9.6942

>T61714 WP_079891856.1 NZ_CP014700:c3486641-3486300 [Bacillus amyloliquefaciens]
IKEALRNNNYEKLTPKETAKHRSKFTSSLKDRLIAEWEEKTNQKWPRYTEEVFDKHGEVARSIGQPYDAHHIIENNFGGP
HEWWNIHPAKYPDEHQAGIHGKGAPSGKLFPRR

Nucleotide

• Length: 342 bp

>T61714 NZ_CP014700:c3486641-3486300 [Bacillus amyloliquefaciens]
ATAAAAGAAGCTTTAAGAAATAATAACTATGAGAAATTAACACCAAAAGAAACAGCTAAGCATAGGAGCAAATTCACATC
AAGTTTGAAAGATAGATTGATTGCAGAATGGGAAGAAAAAACCAATCAAAAGTGGCCTAGATATACAGAAGAGGTTTTTG
ATAAGCATGGAGAAGTTGCCCGAAGTATTGGTCAACCGTACGATGCCCATCATATCATCGAGAACAACTTTGGTGGCCCT
CACGAATGGTGGAATATTCATCCGGCAAAATATCCGGATGAACACCAAGCGGGTATACATGGAAAGGGAGCACCATCAGG
AAAACTTTTTCCAAGGAGGTAA

Antitoxin

Protein

• Length: 146 a.a.
• Molecular weight: 16992.02 Da
• Isoelectric Point: 3.8929

>AT61714 WP_015387595.1 NZ_CP014700:c3486290-3485853 [Bacillus amyloliquefaciens]
MAEFEFINHLENKTYKVPEEDILKAEQRMNVDIPNDLKQLYLEVGYGFIKGESDNAINRILGPGAVADIRLREGIFEFDP
DLGELYDDENKLIFFEVNEGVYISIDLRLANNPIFYFDTQIAESLEDFFKKFLNDNEYYIDLIED

Nucleotide

• Length: 438 bp

>AT61714 NZ_CP014700:c3486290-3485853 [Bacillus amyloliquefaciens]
ATGGCTGAATTTGAATTTATAAATCATTTAGAAAATAAAACATATAAGGTACCTGAAGAGGATATATTAAAAGCTGAACA
AAGAATGAACGTAGATATTCCAAATGATTTAAAACAATTATATTTAGAAGTCGGTTATGGTTTTATTAAAGGTGAAAGTG
ATAATGCAATTAATAGGATATTGGGGCCAGGGGCAGTAGCCGACATTAGACTAAGAGAAGGAATTTTTGAGTTTGACCCA
GATTTAGGTGAACTTTATGATGATGAAAATAAACTTATTTTTTTTGAAGTAAACGAGGGTGTTTATATTTCTATAGATTT
ACGATTGGCAAATAATCCAATATTTTATTTTGACACTCAAATAGCTGAATCTTTAGAGGATTTTTTTAAAAAGTTCCTCA
ATGATAATGAATATTATATTGATTTAATTGAGGATTAG