Detailed information of TA system TA061713

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2489855..2490492
• Replicon: chromosome
• Accession: NZ_CP014700
• Organism: Bacillus amyloliquefaciens strain S499

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: AS588_RS11800
• Protein ID: WP_003156187.1
• Coordinates: 2489855..2490205 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: AS588_RS11805
• Protein ID: WP_003156188.1
• Coordinates: 2490211..2490492 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AS588_RS11760	2484895..2485497	-	603	WP_003156170.1	SpoIIE family protein phosphatase	-
AS588_RS11765	2485497..2486285	-	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
AS588_RS11770	2486251..2486733	-	483	WP_003156173.1	anti-sigma B factor RsbW	-
AS588_RS11775	2486730..2487059	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
AS588_RS11780	2487123..2488130	-	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
AS588_RS11785	2488142..2488543	-	402	WP_003156178.1	anti-sigma regulatory factor	-
AS588_RS11790	2488546..2488911	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
AS588_RS11795	2488916..2489737	-	822	WP_003156182.1	STAS domain-containing protein	-
AS588_RS11800	2489855..2490205	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
AS588_RS11805	2490211..2490492	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
AS588_RS11810	2490612..2491781	-	1170	WP_015388675.1	alanine racemase	-
AS588_RS11815	2491898..2492905	-	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
AS588_RS11820	2493070..2493435	-	366	WP_014304402.1	holo-ACP synthase	-
AS588_RS11825	2493528..2494127	+	600	WP_015388676.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T61713 WP_003156187.1 NZ_CP014700:c2490205-2489855 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T61713 NZ_CP014700:c2490205-2489855 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT61713 WP_003156188.1 NZ_CP014700:c2490492-2490211 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT61713 NZ_CP014700:c2490492-2490211 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5