61712

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1957407..1958324 Replicon chromosome
Accession NZ_CP014700
Organism Bacillus amyloliquefaciens strain S499

Toxin (Protein)


Gene name spoIISA Uniprot ID A0A6A8LGT8
Locus tag AS588_RS08870 Protein ID WP_003154806.1
Coordinates 1957407..1958153 (+) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag AS588_RS08875 Protein ID WP_003154807.1
Coordinates 1958154..1958324 (+) Length 57 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AS588_RS08850 1952798..1953748 - 951 WP_015388352.1 ring-cleaving dioxygenase -
AS588_RS08855 1954072..1955388 + 1317 WP_003154801.1 amino acid permease -
AS588_RS08860 1955674..1956291 + 618 WP_003154804.1 DUF47 domain-containing protein -
AS588_RS08865 1956304..1957302 + 999 WP_003154805.1 inorganic phosphate transporter -
AS588_RS08870 1957407..1958153 + 747 WP_003154806.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
AS588_RS08875 1958154..1958324 + 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
AS588_RS08880 1958582..1959460 - 879 WP_014304858.1 N-acetylmuramoyl-L-alanine amidase -
AS588_RS08885 1959474..1959737 - 264 WP_003154813.1 phage holin -
AS588_RS08890 1959751..1960014 - 264 WP_003154815.1 hemolysin XhlA family protein -
AS588_RS08895 1960066..1960827 - 762 WP_014304857.1 hypothetical protein -
AS588_RS08900 1960884..1961081 - 198 WP_003154819.1 XkdX family protein -
AS588_RS08905 1961087..1961458 - 372 WP_014304856.1 hypothetical protein -
AS588_RS08910 1961471..1963093 - 1623 WP_014304855.1 pyocin knob domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-239)

Antitoxin

(1-56)


Sequences


Toxin        


Download         Length: 249 a.a.        Molecular weight: 29034.50 Da        Isoelectric Point: 4.6947

>T61712 WP_003154806.1 NZ_CP014700:1957407-1958153 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T61712 NZ_CP014700:1957407-1958153 [Bacillus amyloliquefaciens]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
AGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTGGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGGAGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTTCAGGACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT61712 WP_003154807.1 NZ_CP014700:1958154-1958324 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT61712 NZ_CP014700:1958154-1958324 [Bacillus amyloliquefaciens]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGATAAGTATAAACAAA
AAAAAGCTTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A6A8LGT8


Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References