Detailed information of TA system TA061690

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 719..1347
• Replicon: plasmid pKB16680_1
• Accession: NZ_CP014682
• Organism: Kozakia baliensis strain NBRC 16680

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A0U90_RS13120
• Protein ID: WP_003630720.1
• Coordinates: 943..1347 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C7JJ36
• Locus tag: A0U90_RS13115
• Protein ID: WP_012813340.1
• Coordinates: 719..943 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A0U90_RS13115	719..943	+	225	WP_012813340.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
A0U90_RS13120	943..1347	+	405	WP_003630720.1	tRNA(fMet)-specific endonuclease VapC	Toxin
A0U90_RS14990	1555..1755	+	201	WP_012813343.1	type II toxin-antitoxin system CcdA family antitoxin	-
A0U90_RS13125	1756..2061	+	306	WP_012813344.1	CcdB family protein	-
A0U90_RS13130	2337..3332	+	996	WP_070406036.1	restriction endonuclease	-
A0U90_RS13135	3406..4671	+	1266	WP_070406037.1	SIR2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..135727	135727

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (9-46)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15152.26 Da
• Isoelectric Point: 6.0964

>T61690 WP_003630720.1 NZ_CP014682:943-1347 [Kozakia baliensis]
MLRYLLDTNFCIRVLRDRPQGLRERFNDNAEALCISDVVLYELLYGAEKSHDPAKIRREVEHFAARLSVLPFDDDAAAHT
AEIRAELEKRGCVIGPYDLMIAGHARSRGLVVVTGNLRELTRVDGLRSEDWTAG

Nucleotide

• Length: 405 bp

>T61690 NZ_CP014682:943-1347 [Kozakia baliensis]
ATGCTTCGTTATCTTCTGGATACAAACTTTTGCATTCGTGTCCTGCGTGATCGTCCGCAAGGCTTGCGTGAGCGTTTCAA
TGATAACGCCGAGGCGCTCTGCATCTCGGATGTCGTGCTTTACGAACTTCTCTATGGAGCCGAAAAATCTCACGATCCGG
CAAAGATTCGACGTGAGGTTGAGCATTTTGCCGCACGTCTGTCTGTTTTGCCGTTTGATGATGACGCCGCAGCCCATACA
GCGGAAATCAGGGCAGAGCTGGAAAAGCGTGGCTGTGTCATCGGCCCATACGATTTGATGATTGCCGGGCACGCTCGAAG
CAGGGGGCTTGTCGTTGTGACTGGAAACTTACGAGAACTTACGAGGGTAGATGGGTTGCGATCTGAGGATTGGACGGCCG
GTTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8310.55 Da
• Isoelectric Point: 8.9793

>AT61690 WP_012813340.1 NZ_CP014682:719-943 [Kozakia baliensis]
MLTRTTLFLSNRSQAVRLPKAVAFDVNLKDVVIIPDGARRIIAPANAAWDDFFAAPGTPLPERDQPRMQERESF

Nucleotide

• Length: 225 bp

>AT61690 NZ_CP014682:719-943 [Kozakia baliensis]
ATGCTCACCCGCACGACGCTCTTTCTGTCCAATCGCTCCCAGGCCGTGCGTCTTCCCAAAGCGGTTGCATTTGACGTCAA
CCTGAAAGACGTCGTGATCATCCCGGACGGTGCGCGTCGTATAATCGCACCTGCAAATGCCGCATGGGACGATTTCTTTG
CCGCGCCGGGCACTCCACTGCCTGAGCGTGATCAGCCGAGGATGCAGGAACGCGAAAGCTTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A511FS67