Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 7452..8122 Replicon plasmid pKB14400_6
Accession NZ_CP014680
Organism Kozakia baliensis strain DSM 14400

Toxin (Protein)


Gene name vapC Uniprot ID A0A511FSG3
Locus tag A0U89_RS16690 Protein ID WP_045543271.1
Coordinates 7452..7874 (-) Length 141 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A511FSE0
Locus tag A0U89_RS16695 Protein ID WP_045543272.1
Coordinates 7871..8122 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A0U89_RS16665 4499..4984 + 486 Protein_4 IS5-like element IS12528 family transposase -
A0U89_RS17340 5065..6127 + 1063 Protein_5 IS630 family transposase -
A0U89_RS16680 6142..7185 + 1044 Protein_6 transposase -
A0U89_RS16685 7219..7452 - 234 Protein_7 hypothetical protein -
A0U89_RS16690 7452..7874 - 423 WP_045543271.1 type II toxin-antitoxin system VapC family toxin Toxin
A0U89_RS16695 7871..8122 - 252 WP_045543272.1 plasmid stabilization protein Antitoxin
A0U89_RS16700 8188..11273 - 3086 Protein_10 Ti-type conjugative transfer relaxase TraA -
A0U89_RS16705 11437..11730 + 294 WP_070404355.1 conjugal transfer protein TraD -
A0U89_RS16710 11795..12061 + 267 WP_070404347.1 conjugal transfer protein TraD -
A0U89_RS16715 12054..12557 + 504 WP_070404348.1 MucR family transcriptional regulator -
A0U89_RS16720 12588..13103 - 516 WP_045543227.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..30533 30533


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-122)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 141 a.a.        Molecular weight: 15247.54 Da        Isoelectric Point: 5.2273

>T61689 WP_045543271.1 NZ_CP014680:c7874-7452 [Kozakia baliensis]
MILLDTNVISEPWKPAPEPRVLAWIDAQAIETLFLSAVTVAELRFGIGAMPAGRRRSVLHERLEQQVLPLFEGRVLSFDL
AASQAYAELMVRARSEGRAIGKADGYIAAVAASRGYAVASRDVSPFEAAGVRVLNPWEDV

Download         Length: 423 bp

>T61689 NZ_CP014680:c7874-7452 [Kozakia baliensis]
ATGATCCTTCTTGATACCAATGTAATCTCCGAGCCGTGGAAACCTGCCCCTGAACCGCGTGTCCTGGCATGGATTGATGC
GCAGGCGATCGAGACGCTTTTTCTGTCTGCCGTCACGGTGGCGGAACTGCGTTTCGGGATCGGGGCGATGCCGGCCGGTC
GGCGGCGTTCGGTGCTTCATGAGCGGCTGGAGCAGCAGGTTCTGCCTCTCTTCGAAGGGCGTGTCCTGTCGTTCGATCTG
GCGGCGTCACAGGCTTATGCTGAGTTGATGGTGCGGGCCAGATCAGAGGGACGGGCGATTGGGAAAGCGGATGGTTATAT
TGCGGCGGTCGCTGCATCCCGTGGTTATGCTGTGGCCAGCCGCGACGTATCGCCTTTCGAGGCGGCCGGTGTGCGGGTTC
TCAATCCGTGGGAGGATGTCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9212.51 Da        Isoelectric Point: 7.2058

>AT61689 WP_045543272.1 NZ_CP014680:c8122-7871 [Kozakia baliensis]
MSSVVIRNLPEETHRALKAQAMLHGRSTEAEIRTILEAAVRPSQRVRLGSLLASIGREAGMREQDVSTLQVRDQTPAEPM
SFE

Download         Length: 252 bp

>AT61689 NZ_CP014680:c8122-7871 [Kozakia baliensis]
ATGTCCTCTGTCGTTATTCGCAATCTTCCGGAAGAGACGCATCGAGCGCTGAAGGCGCAGGCCATGCTGCATGGGCGCAG
CACGGAAGCGGAGATCCGGACCATTCTGGAAGCGGCCGTCCGGCCCTCGCAGCGGGTGCGACTGGGGTCGCTTCTGGCGT
CCATCGGTCGTGAGGCCGGTATGCGTGAACAGGATGTCTCGACCTTGCAGGTTCGCGATCAAACTCCGGCCGAACCGATG
TCCTTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A511FSG3

Antitoxin

Source ID Structure
AlphaFold DB A0A511FSE0

References