Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 4937..5618 Replicon plasmid pKB14400_5
Accession NZ_CP014679
Organism Kozakia baliensis strain DSM 14400

Toxin (Protein)


Gene name vapC Uniprot ID A0A318PNR1
Locus tag A0U89_RS16485 Protein ID WP_034931108.1
Coordinates 4937..5359 (+) Length 141 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1D8UYY7
Locus tag A0U89_RS16490 Protein ID WP_070404323.1
Coordinates 5424..5618 (+) Length 65 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A0U89_RS16460 47..406 - 360 WP_070404321.1 conjugal transfer protein TraD -
A0U89_RS16465 503..3874 + 3372 WP_083278626.1 Ti-type conjugative transfer relaxase TraA -
A0U89_RS16470 3921..4343 + 423 WP_007396294.1 putative toxin-antitoxin system toxin component, PIN family -
A0U89_RS16475 4340..4603 + 264 WP_070404322.1 toxin-antitoxin system HicB family antitoxin -
A0U89_RS16480 4689..4940 + 252 WP_010512463.1 plasmid stabilization protein -
A0U89_RS16485 4937..5359 + 423 WP_034931108.1 type II toxin-antitoxin system VapC family toxin Toxin
A0U89_RS16490 5424..5618 + 195 WP_070404323.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
A0U89_RS16495 5615..6007 + 393 WP_070404324.1 PIN domain nuclease -
A0U89_RS16505 6301..6972 + 672 WP_070404342.1 hypothetical protein -
A0U89_RS16510 7146..7931 - 786 WP_070404326.1 IS21-like element helper ATPase IstB -
A0U89_RS16515 7921..9445 - 1525 Protein_10 IS21 family transposase -
A0U89_RS16520 9700..10443 - 744 Protein_11 DAK2 domain-containing protein -
A0U89_RS17290 10444..10605 - 162 Protein_12 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..35463 35463


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-122)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-47)


Sequences

Toxin        


Download         Length: 141 a.a.        Molecular weight: 15266.54 Da        Isoelectric Point: 5.2375

>T61688 WP_034931108.1 NZ_CP014679:4937-5359 [Kozakia baliensis]
MILLDTNVISEPWKPVPEPRVLAWIDGQAIETLFLSAVTVAELRFGIGAMPSGRRRSVLHERLEQEVLPLFAGRVLSFDL
AASQAYAELMVRARSEGRAIGKADGYIAATAASRGYVVASRDVSPFEAAGVRVLNPWEET

Download         Length: 423 bp

>T61688 NZ_CP014679:4937-5359 [Kozakia baliensis]
ATGATCCTGCTCGATACCAATGTGATCTCGGAGCCGTGGAAGCCCGTCCCGGAGCCGCGTGTTCTGGCCTGGATCGATGG
ACAGGCGATCGAGACGCTTTTCCTGTCGGCCGTCACGGTGGCGGAGCTGCGTTTCGGGATCGGGGCGATGCCGTCTGGTC
GGCGGCGTTCTGTACTGCATGAGCGGCTGGAACAGGAGGTATTGCCCCTGTTCGCGGGGCGTGTCCTGTCGTTCGATCTG
GCGGCGTCTCAGGCCTATGCCGAACTGATGGTGCGGGCCAGATCAGAGGGCCGCGCAATTGGCAAGGCAGATGGCTATAT
TGCAGCAACCGCTGCATCCCGAGGTTATGTCGTGGCCAGCCGCGACGTGTCTCCTTTCGAGGCGGCCGGTGTGCGGGTTC
TCAATCCGTGGGAGGAGACCTGA

Antitoxin


Download         Length: 65 a.a.        Molecular weight: 7238.46 Da        Isoelectric Point: 10.4776

>AT61688 WP_070404323.1 NZ_CP014679:5424-5618 [Kozakia baliensis]
MRTNIIIDDALMADALKASGVKTKKEAVELGLRTLIRLKQQRELRTLRGKLDWQGDLDAMRSDA

Download         Length: 195 bp

>AT61688 NZ_CP014679:5424-5618 [Kozakia baliensis]
ATGCGAACAAACATCATCATTGACGATGCCCTCATGGCTGATGCGCTGAAGGCGTCTGGCGTCAAAACCAAGAAGGAAGC
AGTCGAACTCGGGTTGCGGACGCTGATCAGGCTGAAGCAGCAGCGGGAACTCCGTACCTTGCGGGGAAAGCTCGACTGGC
AGGGTGATCTTGATGCCATGCGTTCTGACGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A318PNR1

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8UYY7

References