Detailed information of TA system TA061685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 83036..83586
• Replicon: plasmid pKB14400_2
• Accession: NZ_CP014676
• Organism: Kozakia baliensis strain DSM 14400

Toxin (Protein)

• Gene name: relE
• Uniprot ID: C7JII7
• Locus tag: A0U89_RS15440
• Protein ID: WP_003618711.1
• Coordinates: 83293..83586 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D5QDQ9
• Locus tag: A0U89_RS15435
• Protein ID: WP_003618686.1
• Coordinates: 83036..83296 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A0U89_RS15400	78321..78959	+	639	WP_070404147.1	tyrosine-type recombinase/integrase	-
A0U89_RS15405	78968..79306	+	339	WP_181015904.1	prevent-host-death protein	-
A0U89_RS15410	79303..79686	+	384	WP_048854875.1	type II toxin-antitoxin system VapC family toxin	-
A0U89_RS17930	80264..80428	+	165	WP_158513598.1	hypothetical protein	-
A0U89_RS15430	81738..82568	-	831	WP_070404170.1	IS5 family transposase	-
A0U89_RS17205	82645..82755	+	111	Protein_92	zinc-binding dehydrogenase	-
A0U89_RS15435	83036..83296	+	261	WP_003618686.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
A0U89_RS15440	83293..83586	+	294	WP_003618711.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
A0U89_RS15445	83718..84980	+	1263	WP_070404149.1	sodium:proton antiporter	-
A0U89_RS15450	84980..85663	+	684	WP_029604387.1	DUF1275 domain-containing protein	-
A0U89_RS15455	86102..86812	+	711	WP_147061376.1	hypothetical protein	-
A0U89_RS15460	87017..87790	-	774	WP_070404151.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..102455	102455

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11605.35 Da
• Isoelectric Point: 7.9998

>T61685 WP_003618711.1 NZ_CP014676:83293-83586 [Kozakia baliensis]
MKEYCLEFDDRALREWDALDGSVRKKFEKKLEKLIWNPHSPGNELHRDLSGFYKIKLLKDGYRLVYQVIDERIVIYVIAV
GRRADNEIYELASKRTE

Nucleotide

• Length: 294 bp

>T61685 NZ_CP014676:83293-83586 [Kozakia baliensis]
ATGAAAGAGTATTGTCTTGAATTCGACGACAGAGCACTCAGAGAGTGGGATGCTCTTGACGGCAGCGTGCGTAAGAAATT
TGAAAAGAAGCTGGAAAAGCTGATCTGGAACCCTCACTCTCCAGGAAACGAACTGCATCGCGACCTATCAGGGTTTTATA
AGATCAAGCTTCTGAAAGATGGTTACCGCCTTGTTTATCAGGTCATCGACGAACGGATCGTCATCTATGTAATTGCCGTT
GGCCGGAGAGCCGATAATGAGATTTATGAACTGGCTTCGAAGCGAACAGAGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9762.23 Da
• Isoelectric Point: 10.4645

>AT61685 WP_003618686.1 NZ_CP014676:83036-83296 [Kozakia baliensis]
MSAVTFRVDETLKAAAVKKLSAQGISLSDALRDTLAYIAETGRSPVKRRLVTDEDAELIEIVRERLKNPAQKHRMTFAEL
KNRHRE

Nucleotide

• Length: 261 bp

>AT61685 NZ_CP014676:83036-83296 [Kozakia baliensis]
ATGTCTGCTGTTACATTCCGGGTAGATGAAACGCTGAAGGCAGCCGCAGTTAAGAAGTTGTCGGCTCAAGGCATTTCACT
TTCTGACGCACTGCGCGATACGCTCGCGTATATTGCTGAAACGGGTCGCTCTCCCGTCAAGCGGAGGCTTGTCACAGACG
AGGATGCGGAGCTTATCGAGATTGTGCGCGAACGCCTAAAAAACCCTGCCCAAAAGCATCGTATGACTTTTGCAGAGCTT
AAGAACCGTCATCGCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6N3T9M6