Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-PrlF
Location 2224260..2224815 Replicon chromosome
Accession NZ_CP014671
Organism Immundisolibacter cernigliae strain TR3.2

Toxin (Protein)


Gene name mazF Uniprot ID A0A1B1YV12
Locus tag PG2T_RS10590 Protein ID WP_068805051.1
Coordinates 2224465..2224815 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1B1YUX1
Locus tag PG2T_RS10585 Protein ID WP_068805048.1
Coordinates 2224260..2224478 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PG2T_RS10555 2220605..2220862 + 258 WP_068805034.1 type II toxin-antitoxin system ParD family antitoxin -
PG2T_RS10560 2220859..2221158 + 300 WP_068805036.1 type II toxin-antitoxin system RelE/ParE family toxin -
PG2T_RS10565 2221297..2222001 + 705 WP_068805039.1 DsbA family protein -
PG2T_RS10570 2222108..2222347 + 240 WP_068805041.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
PG2T_RS10575 2222335..2222649 + 315 WP_068805044.1 type II toxin-antitoxin system PemK/MazF family toxin -
PG2T_RS15555 2222850..2223680 + 831 WP_075968172.1 arsenate reductase (glutaredoxin) -
PG2T_RS10585 2224260..2224478 + 219 WP_068805048.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
PG2T_RS10590 2224465..2224815 + 351 WP_068805051.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
PG2T_RS10595 2224924..2226480 - 1557 WP_068805054.1 GNAT family N-acetyltransferase -
PG2T_RS10600 2226518..2227774 - 1257 WP_202816317.1 MFS transporter -
PG2T_RS10605 2227912..2228175 + 264 WP_068805060.1 hypothetical protein -
PG2T_RS10610 2228299..2228898 + 600 WP_068805063.1 TIGR00645 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-109)

Antitoxin

PrlF antitoxin

(4-53)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12258.16 Da        Isoelectric Point: 10.7876

>T61683 WP_068805051.1 NZ_CP014671:2224465-2224815 [Immundisolibacter cernigliae]
MPTFRQGDVIKVPFPYTDRSTRQSRPALVVSAGSLEEAHGLLWVVMITSAQNRGWPGDVPVGNLAAAGLPVPSVIRAAKI
ATIEATDASRLGTVAPAVFRRVAQCLANQLGLDARP

Download         Length: 351 bp

>T61683 NZ_CP014671:2224465-2224815 [Immundisolibacter cernigliae]
ATGCCGACCTTTAGGCAGGGCGACGTCATCAAGGTGCCGTTTCCGTACACGGACCGCTCCACCCGGCAGTCCAGGCCAGC
ACTGGTGGTGTCGGCCGGCAGCCTCGAAGAGGCCCACGGACTGCTGTGGGTGGTCATGATCACCAGCGCTCAGAACCGGG
GATGGCCGGGCGACGTGCCGGTGGGCAATCTTGCGGCAGCCGGCCTACCGGTGCCGTCCGTGATCCGGGCCGCGAAAATC
GCCACCATCGAAGCGACGGACGCGTCCAGGTTGGGTACTGTGGCCCCCGCGGTATTCCGACGGGTGGCCCAATGTCTGGC
CAATCAGCTTGGTCTGGACGCCCGACCGTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8123.10 Da        Isoelectric Point: 4.5238

>AT61683 WP_068805048.1 NZ_CP014671:2224260-2224478 [Immundisolibacter cernigliae]
MISSKLTSKAQTTIPQPVRVALHLEPGDELVYEIDDQRVILTKAKTSDRADDPFRTFQEWSTEADAKAYADL

Download         Length: 219 bp

>AT61683 NZ_CP014671:2224260-2224478 [Immundisolibacter cernigliae]
ATGATCAGCAGCAAACTGACCAGCAAAGCGCAGACGACCATCCCGCAACCCGTTCGTGTTGCCCTGCACCTGGAGCCAGG
CGACGAATTGGTCTACGAGATTGATGATCAGCGCGTGATTCTTACCAAGGCCAAAACCAGCGACAGGGCTGACGATCCAT
TCCGCACGTTTCAGGAGTGGAGCACCGAAGCGGACGCGAAGGCTTATGCCGACCTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B1YV12

Antitoxin

Source ID Structure
AlphaFold DB A0A1B1YUX1

References