Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 39017..39542 Replicon plasmid pKpQIL-571
Accession NZ_CP014669
Organism Escherichia coli strain ECONIH2

Toxin (Protein)


Gene name ccdB Uniprot ID W8UQV6
Locus tag WM48_RS25930 Protein ID WP_001568026.1
Coordinates 39237..39542 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag WM48_RS25925 Protein ID WP_001568025.1
Coordinates 39017..39235 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WM48_RS25885 34407..34610 - 204 WP_004197809.1 hypothetical protein -
WM48_RS25890 34624..34827 - 204 WP_004197808.1 hemolysin expression modulator Hha -
WM48_RS25895 34861..35229 - 369 WP_004197807.1 hypothetical protein -
WM48_RS25900 35273..35767 - 495 WP_020805591.1 hypothetical protein -
WM48_RS25905 35798..36370 - 573 WP_020805590.1 hypothetical protein -
WM48_RS25910 36367..36615 - 249 WP_000272716.1 hypothetical protein -
WM48_RS25915 37052..37741 + 690 WP_020315560.1 RES family NAD+ phosphorylase -
WM48_RS25920 37773..38462 - 690 WP_004193995.1 hypothetical protein -
WM48_RS29530 38602..38847 + 246 Protein_41 hypothetical protein -
WM48_RS25925 39017..39235 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
WM48_RS25930 39237..39542 + 306 WP_001568026.1 type II toxin-antitoxin system toxin CcdB Toxin
WM48_RS25935 39711..40106 + 396 WP_072158608.1 hypothetical protein -
WM48_RS29535 40133..40456 + 324 WP_022644730.1 hypothetical protein -
WM48_RS25945 40453..41469 + 1017 WP_020315256.1 hypothetical protein -
WM48_RS25950 41804..43072 - 1269 WP_019705993.1 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-3 - 1..113852 113852
- flank IS/Tn - - 41804..43072 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11645.32 Da        Isoelectric Point: 5.6919

>T61677 WP_001568026.1 NZ_CP014669:39237-39542 [Escherichia coli]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T61677 NZ_CP014669:39237-39542 [Escherichia coli]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCTGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT61677 WP_001568025.1 NZ_CP014669:39017-39235 [Escherichia coli]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT61677 NZ_CP014669:39017-39235 [Escherichia coli]
ATGAAGCAGCGCATCACTGTCACCATAGACAGTGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EZN1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References