Detailed information of TA system TA061671

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4968170..4969005
• Replicon: chromosome
• Accession: NZ_CP014667
• Organism: Escherichia coli strain ECONIH2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q1R2L4
• Locus tag: WM48_RS24265
• Protein ID: WP_001094426.1
• Coordinates: 4968628..4969005 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: Q1R2L5
• Locus tag: WM48_RS24260
• Protein ID: WP_001285575.1
• Coordinates: 4968170..4968538 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM48_RS24225	4964063..4964743	+	681	WP_001282919.1	WYL domain-containing protein	-
WM48_RS24230	4964891..4965568	+	678	WP_001097301.1	hypothetical protein	-
WM48_RS24235	4965574..4965807	+	234	WP_001278290.1	DUF905 family protein	-
WM48_RS24240	4965897..4966715	+	819	WP_001175160.1	DUF945 domain-containing protein	-
WM48_RS24245	4966806..4967291	+	486	WP_065089985.1	antirestriction protein	-
WM48_RS24250	4967306..4967782	+	477	WP_001298074.1	RadC family protein	-
WM48_RS24255	4967869..4968090	+	222	WP_000692350.1	DUF987 domain-containing protein	-
WM48_RS24260	4968170..4968538	+	369	WP_001285575.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
WM48_RS24265	4968628..4969005	+	378	WP_001094426.1	TA system toxin CbtA family protein	Toxin
WM48_RS24270	4969002..4969490	+	489	WP_000761683.1	hypothetical protein	-
WM48_RS24275	4969502..4969699	+	198	WP_000839272.1	DUF957 domain-containing protein	-
WM48_RS29735	4969778..4969936	+	159	WP_001467148.1	hypothetical protein	-
WM48_RS24280	4970888..4972924	+	2037	WP_000417001.1	hypothetical protein	-
WM48_RS24285	4973323..4973502	-	180	WP_001298063.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14072.99 Da
• Isoelectric Point: 7.3523

>T61671 WP_001094426.1 NZ_CP014667:4968628-4969005 [Escherichia coli]
MNTLPDTHIREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T61671 NZ_CP014667:4968628-4969005 [Escherichia coli]
ATGAACACATTACCCGATACACACATACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13709.52 Da
• Isoelectric Point: 6.6258

>AT61671 WP_001285575.1 NZ_CP014667:4968170-4968538 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT61671 NZ_CP014667:4968170-4968538 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A454ABD6

Antitoxin

• AlphaFold DB: A0A454ABF5