Detailed information of TA system TA061665

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3353049..3353881
• Replicon: chromosome
• Accession: NZ_CP014667
• Organism: Escherichia coli strain ECONIH2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: WM48_RS16405
• Protein ID: WP_000854753.1
• Coordinates: 3353507..3353881 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: WM48_RS16400
• Protein ID: WP_001531802.1
• Coordinates: 3353049..3353417 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM48_RS16365	3349883..3350338	+	456	WP_000581502.1	hypothetical protein	-
WM48_RS29700	3350457..3350668	-	212	Protein_3132	hypothetical protein	-
WM48_RS16375	3350749..3351570	+	822	WP_001531953.1	DUF945 domain-containing protein	-
WM48_RS16380	3351625..3352110	+	486	WP_001531954.1	antirestriction protein	-
WM48_RS16385	3352126..3352602	+	477	WP_001186726.1	RadC family protein	-
WM48_RS16390	3352665..3352886	+	222	WP_000692329.1	DUF987 domain-containing protein	-
WM48_RS16400	3353049..3353417	+	369	WP_001531802.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
WM48_RS16405	3353507..3353881	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
WM48_RS16410	3353878..3354366	+	489	WP_000777541.1	hypothetical protein	-
WM48_RS16415	3354378..3354575	+	198	WP_000839281.1	DUF957 domain-containing protein	-
WM48_RS16420	3354672..3355241	+	570	WP_001290247.1	DUF4942 domain-containing protein	-
WM48_RS16425	3355583..3355753	+	171	Protein_3142	IS110 family transposase	-
WM48_RS16430	3356563..3357546	+	984	WP_001335608.1	KpsF/GutQ family sugar-phosphate isomerase	-
WM48_RS16435	3357618..3358766	+	1149	WP_000905914.1	capsule polysaccharide transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3355649..3355753	104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T61665 WP_000854753.1 NZ_CP014667:3353507-3353881 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T61665 NZ_CP014667:3353507-3353881 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13422.27 Da
• Isoelectric Point: 6.3161

>AT61665 WP_001531802.1 NZ_CP014667:3353049-3353417 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNSLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT61665 NZ_CP014667:3353049-3353417 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACAGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0