Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3209533..3210187 Replicon chromosome
Accession NZ_CP014667
Organism Escherichia coli strain ECONIH2

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag WM48_RS15645 Protein ID WP_000244765.1
Coordinates 3209533..3209940 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag WM48_RS15650 Protein ID WP_000354046.1
Coordinates 3209921..3210187 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WM48_RS15625 3205490..3207223 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
WM48_RS15630 3207229..3207939 - 711 WP_000715229.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
WM48_RS15635 3207964..3208860 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
WM48_RS15640 3208972..3209493 + 522 WP_001055874.1 flavodoxin FldB -
WM48_RS15645 3209533..3209940 - 408 WP_000244765.1 protein YgfX Toxin
WM48_RS15650 3209921..3210187 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
WM48_RS15655 3210430..3211410 + 981 WP_000886080.1 tRNA-modifying protein YgfZ -
WM48_RS15660 3211487..3212146 - 660 WP_000250274.1 hemolysin III family protein -
WM48_RS15665 3212310..3212621 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
WM48_RS15670 3212666..3214099 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T61664 WP_000244765.1 NZ_CP014667:c3209940-3209533 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T61664 NZ_CP014667:c3209940-3209533 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT61664 WP_000354046.1 NZ_CP014667:c3210187-3209921 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT61664 NZ_CP014667:c3210187-3209921 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References