Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 554966..555584 Replicon chromosome
Accession NZ_CP014667
Organism Escherichia coli strain ECONIH2

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag WM48_RS02625 Protein ID WP_001291435.1
Coordinates 554966..555184 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag WM48_RS02630 Protein ID WP_000344800.1
Coordinates 555210..555584 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WM48_RS02590 550253..550825 + 573 WP_000779830.1 YbaY family lipoprotein -
WM48_RS02595 550856..551167 - 312 WP_000409908.1 MGMT family protein -
WM48_RS02605 551546..551899 + 354 WP_000878138.1 DUF1428 family protein -
WM48_RS02610 551941..553491 - 1551 WP_001298569.1 cyclic-guanylate-specific phosphodiesterase PdeB -
WM48_RS02615 553655..554125 - 471 WP_000136192.1 YlaC family protein -
WM48_RS02620 554241..554792 - 552 WP_000102539.1 maltose O-acetyltransferase -
WM48_RS02625 554966..555184 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
WM48_RS02630 555210..555584 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
WM48_RS02635 556129..559278 - 3150 WP_001132481.1 multidrug efflux RND transporter permease subunit -
WM48_RS02640 559301..560494 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T61649 WP_001291435.1 NZ_CP014667:c555184-554966 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T61649 NZ_CP014667:c555184-554966 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT61649 WP_000344800.1 NZ_CP014667:c555584-555210 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT61649 NZ_CP014667:c555584-555210 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References