Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 51932..52482 Replicon chromosome
Accession NZ_CP014667
Organism Escherichia coli strain ECONIH2

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag WM48_RS00265 Protein ID WP_001160966.1
Coordinates 52168..52482 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag WM48_RS00260 Protein ID WP_000125568.1
Coordinates 51932..52165 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WM48_RS00235 47005..47292 + 288 WP_000203741.1 ferredoxin-like protein FixX -
WM48_RS00240 47351..48682 + 1332 WP_001183198.1 MFS transporter -
WM48_RS00245 48790..49320 + 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
WM48_RS00250 49313..51175 + 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
WM48_RS00255 51367..51846 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
WM48_RS00260 51932..52165 + 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
WM48_RS00265 52168..52482 + 315 WP_001160966.1 CcdB family protein Toxin
WM48_RS00270 52479..53327 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
WM48_RS00275 53334..53711 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
WM48_RS00280 53714..54535 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
WM48_RS00285 54532..55521 - 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
WM48_RS00290 55521..56807 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T61646 WP_001160966.1 NZ_CP014667:52168-52482 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T61646 NZ_CP014667:52168-52482 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT61646 WP_000125568.1 NZ_CP014667:51932-52165 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT61646 NZ_CP014667:51932-52165 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References