Detailed information of TA system TA061640

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 121501..122237
• Replicon: plasmid pKPN-fff
• Accession: NZ_CP014649
• Organism: Klebsiella pneumoniae strain KPNIH36

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: ABA76_RS28250
• Protein ID: WP_003026803.1
• Coordinates: 121755..122237 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: ABA76_RS28245
• Protein ID: WP_003026799.1
• Coordinates: 121501..121767 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ABA76_RS28200	117563..117925	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
ABA76_RS28205	117975..118325	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
ABA76_RS28210	118683..118952	+	270	WP_004152102.1	hypothetical protein	-
ABA76_RS28215	118940..119515	+	576	WP_004152103.1	hypothetical protein	-
ABA76_RS28220	119546..120040	+	495	WP_004152104.1	DNA-binding protein	-
ABA76_RS28225	120084..120452	+	369	WP_004152105.1	hypothetical protein	-
ABA76_RS28230	120486..120689	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
ABA76_RS28235	120738..120995	+	258	WP_004152107.1	hypothetical protein	-
ABA76_RS28240	121071..121325	+	255	WP_004152108.1	hypothetical protein	-
ABA76_RS28245	121501..121767	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
ABA76_RS28250	121755..122237	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
ABA76_RS28255	122449..123795	+	1347	WP_020314316.1	ISNCY family transposase	-
ABA76_RS31280	124296..125550	-	1255	Protein_144	IS3 family transposase	-
ABA76_RS28270	125638..126600	-	963	WP_004152113.1	M48 family metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..133484	133484

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T61640 WP_003026803.1 NZ_CP014649:121755-122237 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T61640 NZ_CP014649:121755-122237 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT61640 WP_003026799.1 NZ_CP014649:121501-121767 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT61640 NZ_CP014649:121501-121767 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66