Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 121501..122237 Replicon plasmid pKPN-fff
Accession NZ_CP014649
Organism Klebsiella pneumoniae strain KPNIH36

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag ABA76_RS28250 Protein ID WP_003026803.1
Coordinates 121755..122237 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag ABA76_RS28245 Protein ID WP_003026799.1
Coordinates 121501..121767 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ABA76_RS28200 117563..117925 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
ABA76_RS28205 117975..118325 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
ABA76_RS28210 118683..118952 + 270 WP_004152102.1 hypothetical protein -
ABA76_RS28215 118940..119515 + 576 WP_004152103.1 hypothetical protein -
ABA76_RS28220 119546..120040 + 495 WP_004152104.1 DNA-binding protein -
ABA76_RS28225 120084..120452 + 369 WP_004152105.1 hypothetical protein -
ABA76_RS28230 120486..120689 + 204 WP_004152106.1 hemolysin expression modulator Hha -
ABA76_RS28235 120738..120995 + 258 WP_004152107.1 hypothetical protein -
ABA76_RS28240 121071..121325 + 255 WP_004152108.1 hypothetical protein -
ABA76_RS28245 121501..121767 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
ABA76_RS28250 121755..122237 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
ABA76_RS28255 122449..123795 + 1347 WP_020314316.1 ISNCY family transposase -
ABA76_RS31280 124296..125550 - 1255 Protein_144 IS3 family transposase -
ABA76_RS28270 125638..126600 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..133484 133484


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T61640 WP_003026803.1 NZ_CP014649:121755-122237 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T61640 NZ_CP014649:121755-122237 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT61640 WP_003026799.1 NZ_CP014649:121501-121767 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT61640 NZ_CP014649:121501-121767 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References