Detailed information of TA system TA061620

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 2405978..2406510
• Replicon: chromosome
• Accession: NZ_CP014646
• Organism: Thauera humireducens strain SgZ-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AC731_RS11265
• Protein ID: WP_048706114.1
• Coordinates: 2405978..2406301 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AC731_RS11270
• Protein ID: WP_048706118.1
• Coordinates: 2406298..2406510 (-)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AC731_RS11250	2402118..2403476	-	1359	WP_048710044.1	phenylacetate--CoA ligase family protein	-
AC731_RS11255	2403482..2404735	-	1254	WP_048706110.1	glycosyltransferase	-
AC731_RS11260	2404728..2405942	-	1215	WP_048706112.1	glycosyltransferase, exosortase A system-associated	-
AC731_RS11265	2405978..2406301	-	324	WP_048706114.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AC731_RS11270	2406298..2406510	-	213	WP_048706118.1	antitoxin MazE family protein	Antitoxin
AC731_RS11275	2406542..2408494	-	1953	WP_048706120.1	amidotransferase 1, exosortase A system-associated	-
AC731_RS11280	2408510..2409685	-	1176	WP_048706122.1	TIGR03088 family PEP-CTERM/XrtA system glycosyltransferase	-
AC731_RS11285	2409682..2411202	-	1521	WP_048706124.1	exosortase A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2378913..2406510	27597

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-106)

Antitoxin

• Antitoxin MazE-like (7-69)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11796.67 Da
• Isoelectric Point: 5.8213

>T61620 WP_048706114.1 NZ_CP014646:c2406301-2405978 [Thauera humireducens]
VMRGDLITVALQGDYGKPRPAVVVQSDLFDEHPSVTLLPLTSDLRDTPLFRITIEPSPENGLRQRSQIMVDKIQTVTKEK
IGPSLGRLSDEAQIAVNRSLMLFLGLA

Nucleotide

• Length: 324 bp

>T61620 NZ_CP014646:c2406301-2405978 [Thauera humireducens]
GTGATGCGTGGCGATCTGATTACCGTCGCCTTGCAGGGCGACTATGGCAAACCCCGTCCAGCCGTGGTCGTTCAGTCAGA
CTTGTTCGACGAGCACCCGTCGGTCACGCTCCTGCCGCTGACCAGTGATTTACGCGACACACCGCTGTTTCGCATCACCA
TCGAGCCATCACCAGAGAATGGCCTGCGTCAGCGATCACAGATCATGGTTGACAAGATTCAGACTGTGACCAAGGAGAAG
ATCGGTCCCAGCCTGGGCCGGTTGTCTGATGAAGCACAGATTGCTGTCAATCGGTCCTTGATGTTGTTCCTAGGGCTGGC
TTGA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 8029.02 Da
• Isoelectric Point: 5.0806

>AT61620 WP_048706118.1 NZ_CP014646:c2406510-2406298 [Thauera humireducens]
MAASVSERVGRYRTSLREAGLRPVQIWVPDTRKPGFAEECRRQSSLLKGDPQEQEVLDWLERVADTDGWV

Nucleotide

• Length: 213 bp

>AT61620 NZ_CP014646:c2406510-2406298 [Thauera humireducens]
ATGGCTGCCAGTGTTTCTGAGCGTGTTGGGCGCTACCGCACAAGTCTTCGGGAGGCAGGCTTGCGCCCGGTCCAGATCTG
GGTGCCAGACACGCGCAAGCCGGGTTTTGCCGAGGAGTGCCGCAGGCAGTCCAGTTTGCTGAAGGGCGATCCGCAGGAGC
AGGAAGTGCTCGACTGGCTCGAGCGGGTCGCTGACACGGACGGCTGGGTGTGA