Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2303860..2304514 Replicon chromosome
Accession NZ_CP014642
Organism Escherichia coli strain BLK16

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag AYR48_RS11640 Protein ID WP_000244783.1
Coordinates 2303860..2304267 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AYR48_RS11645 Protein ID WP_000354046.1
Coordinates 2304248..2304514 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYR48_RS11620 (2299817) 2299817..2301550 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
AYR48_RS11625 (2301556) 2301556..2302266 - 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AYR48_RS11630 (2302291) 2302291..2303187 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AYR48_RS11635 (2303299) 2303299..2303820 + 522 WP_001055874.1 flavodoxin FldB -
AYR48_RS11640 (2303860) 2303860..2304267 - 408 WP_000244783.1 protein YgfX Toxin
AYR48_RS11645 (2304248) 2304248..2304514 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AYR48_RS11650 (2304757) 2304757..2305737 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
AYR48_RS11655 (2305933) 2305933..2306592 - 660 WP_000250274.1 hemolysin III family protein -
AYR48_RS11660 (2306756) 2306756..2307067 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
AYR48_RS11665 (2307112) 2307112..2308545 + 1434 WP_012775976.1 6-phospho-beta-glucosidase BglA -
AYR48_RS11670 (2308602) 2308602..2309345 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T61613 WP_000244783.1 NZ_CP014642:c2304267-2303860 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T61613 NZ_CP014642:c2304267-2303860 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTAGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT61613 WP_000354046.1 NZ_CP014642:c2304514-2304248 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT61613 NZ_CP014642:c2304514-2304248 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References