Detailed information of TA system TA061599

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 62598..63435
• Replicon: chromosome
• Accession: NZ_CP014642
• Organism: Escherichia coli strain BLK16

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A140NB16
• Locus tag: AYR48_RS00315
• Protein ID: WP_000227786.1
• Coordinates: 62598..63140 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: AYR48_RS00320
• Protein ID: WP_001297137.1
• Coordinates: 63124..63435 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR48_RS00295 (58128)	58128..59039	-	912	WP_000705853.1	2-dehydropantoate 2-reductase	-
AYR48_RS00300 (59207)	59207..59698	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
AYR48_RS00305 (59826)	59826..61190	-	1365	WP_001000978.1	MFS transporter	-
AYR48_RS00310 (61607)	61607..62542	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
AYR48_RS00315 (62598)	62598..63140	-	543	WP_000227786.1	GNAT family N-acetyltransferase	Toxin
AYR48_RS00320 (63124)	63124..63435	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
AYR48_RS00325 (63620)	63620..64510	-	891	WP_000971336.1	heme o synthase	-
AYR48_RS00330 (64522)	64522..64851	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AYR48_RS00335 (64851)	64851..65465	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AYR48_RS00340 (65455)	65455..67446	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AYR48_RS00345 (67468)	67468..68415	-	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19794.98 Da
• Isoelectric Point: 8.3395

>T61599 WP_000227786.1 NZ_CP014642:c63140-62598 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T61599 NZ_CP014642:c63140-62598 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACTCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT61599 WP_001297137.1 NZ_CP014642:c63435-63124 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT61599 NZ_CP014642:c63435-63124 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A140NB16

Antitoxin

• AlphaFold DB: A0A0E0Y6B3