Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3629131..3629628 Replicon chromosome
Accession NZ_CP014641
Organism Escherichia coli strain BLK9

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AYL54_RS18455 Protein ID WP_001173343.1
Coordinates 3629455..3629628 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag AYL54_RS18450 Protein ID WP_000398619.1
Coordinates 3629131..3629427 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYL54_RS18425 (3625857) 3625857..3626576 - 720 WP_000611288.1 two-component system response regulator DcuR -
AYL54_RS18430 (3626573) 3626573..3628209 - 1637 Protein_3381 sensor histidine kinase -
AYL54_RS18435 (3628390) 3628390..3628620 + 231 WP_001070602.1 4Fe-4S mono-cluster protein YjdI -
AYL54_RS18440 (3628632) 3628632..3628904 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
AYL54_RS18450 (3629131) 3629131..3629427 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AYL54_RS18455 (3629455) 3629455..3629628 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AYL54_RS18460 (3629747) 3629747..3631264 - 1518 WP_001295090.1 lysine--tRNA ligase -
AYL54_RS18465 (3631501) 3631501..3632958 - 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T61596 WP_001173343.1 NZ_CP014641:3629455-3629628 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T61596 NZ_CP014641:3629455-3629628 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT61596 WP_000398619.1 NZ_CP014641:3629131-3629427 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT61596 NZ_CP014641:3629131-3629427 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References