Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2303726..2304380 Replicon chromosome
Accession NZ_CP014641
Organism Escherichia coli strain BLK9

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag AYL54_RS11630 Protein ID WP_000244783.1
Coordinates 2303726..2304133 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AYL54_RS11635 Protein ID WP_000354046.1
Coordinates 2304114..2304380 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYL54_RS11610 (2299683) 2299683..2301416 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
AYL54_RS11615 (2301422) 2301422..2302132 - 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AYL54_RS11620 (2302157) 2302157..2303053 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AYL54_RS11625 (2303165) 2303165..2303686 + 522 WP_001055874.1 flavodoxin FldB -
AYL54_RS11630 (2303726) 2303726..2304133 - 408 WP_000244783.1 protein YgfX Toxin
AYL54_RS11635 (2304114) 2304114..2304380 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AYL54_RS11640 (2304623) 2304623..2305603 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
AYL54_RS11645 (2305799) 2305799..2306458 - 660 WP_000250274.1 hemolysin III family protein -
AYL54_RS11650 (2306622) 2306622..2306933 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
AYL54_RS11655 (2306978) 2306978..2308411 + 1434 WP_012775976.1 6-phospho-beta-glucosidase BglA -
AYL54_RS11660 (2308468) 2308468..2309211 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T61593 WP_000244783.1 NZ_CP014641:c2304133-2303726 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T61593 NZ_CP014641:c2304133-2303726 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTAGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT61593 WP_000354046.1 NZ_CP014641:c2304380-2304114 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT61593 NZ_CP014641:c2304380-2304114 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References