Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 98253..98871 Replicon chromosome
Accession NZ_CP014641
Organism Escherichia coli strain BLK9

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AYL54_RS00500 Protein ID WP_001291435.1
Coordinates 98253..98471 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AYL54_RS00505 Protein ID WP_000344800.1
Coordinates 98497..98871 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYL54_RS00465 (93542) 93542..94114 + 573 WP_000779826.1 YbaY family lipoprotein -
AYL54_RS00470 (94145) 94145..94456 - 312 WP_000409911.1 MGMT family protein -
AYL54_RS00480 (94835) 94835..95188 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AYL54_RS00485 (95230) 95230..96780 - 1551 WP_001319841.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AYL54_RS00490 (96944) 96944..97414 - 471 WP_000136192.1 YlaC family protein -
AYL54_RS00495 (97530) 97530..98081 - 552 WP_000102564.1 maltose O-acetyltransferase -
AYL54_RS00500 (98253) 98253..98471 - 219 WP_001291435.1 HHA domain-containing protein Toxin
AYL54_RS00505 (98497) 98497..98871 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AYL54_RS00510 (99417) 99417..102566 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
AYL54_RS00515 (102589) 102589..103782 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T61580 WP_001291435.1 NZ_CP014641:c98471-98253 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T61580 NZ_CP014641:c98471-98253 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT61580 WP_000344800.1 NZ_CP014641:c98871-98497 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT61580 NZ_CP014641:c98871-98497 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References