Detailed information of TA system TA061579

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 62592..63429
• Replicon: chromosome
• Accession: NZ_CP014641
• Organism: Escherichia coli strain BLK9

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A140NB16
• Locus tag: AYL54_RS00315
• Protein ID: WP_000227786.1
• Coordinates: 62592..63134 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: AYL54_RS00320
• Protein ID: WP_001297137.1
• Coordinates: 63118..63429 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYL54_RS00295 (58122)	58122..59033	-	912	WP_000705853.1	2-dehydropantoate 2-reductase	-
AYL54_RS00300 (59201)	59201..59692	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
AYL54_RS00305 (59820)	59820..61184	-	1365	WP_001000978.1	MFS transporter	-
AYL54_RS00310 (61601)	61601..62536	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
AYL54_RS00315 (62592)	62592..63134	-	543	WP_000227786.1	GNAT family N-acetyltransferase	Toxin
AYL54_RS00320 (63118)	63118..63429	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
AYL54_RS00325 (63614)	63614..64504	-	891	WP_000971336.1	heme o synthase	-
AYL54_RS00330 (64516)	64516..64845	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AYL54_RS00335 (64845)	64845..65459	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AYL54_RS00340 (65449)	65449..67440	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AYL54_RS00345 (67462)	67462..68409	-	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19794.98 Da
• Isoelectric Point: 8.3395

>T61579 WP_000227786.1 NZ_CP014641:c63134-62592 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T61579 NZ_CP014641:c63134-62592 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACTCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT61579 WP_001297137.1 NZ_CP014641:c63429-63118 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT61579 NZ_CP014641:c63429-63118 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A140NB16

Antitoxin

• AlphaFold DB: A0A0E0Y6B3