Detailed information of TA system TA061578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 54267..54938
• Replicon: plasmid pR99
• Accession: NZ_CP014638
• Organism: Vibrio vulnificus strain CECT 4999

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A7DVA5
• Locus tag: VVCECT4999_RS24250
• Protein ID: WP_011988339.1
• Coordinates: 54639..54938 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: VVCECT4999_RS24245
• Protein ID: WP_011988338.1
• Coordinates: 54267..54563 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VVCECT4999_RS24220	49554..50843	+	1290	Protein_32	toprim domain-containing protein	-
VVCECT4999_RS24225	50862..51221	-	360	WP_011988334.1	hypothetical protein	-
VVCECT4999_RS24230	51787..52233	-	447	WP_011988335.1	lytic transglycosylase domain-containing protein	-
VVCECT4999_RS24235	52316..53698	-	1383	WP_011988336.1	DUF3560 domain-containing protein	-
VVCECT4999_RS24240	53896..54162	+	267	WP_011988337.1	hypothetical protein	-
VVCECT4999_RS24245	54267..54563	-	297	WP_011988338.1	putative addiction module antidote protein	Antitoxin
VVCECT4999_RS24250	54639..54938	-	300	WP_011988339.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VVCECT4999_RS24255	54976..55194	-	219	WP_011988340.1	hypothetical protein	-
VVCECT4999_RS24260	55429..55752	-	324	WP_011988342.1	hypothetical protein	-
VVCECT4999_RS24265	55819..56127	-	309	WP_011988343.1	DUF1738 domain-containing protein	-
VVCECT4999_RS24270	56460..57992	+	1533	WP_011988344.1	IS21 family transposase	-
VVCECT4999_RS24275	58004..58744	+	741	WP_011988345.1	IS21-like element ISVch3 family helper ATPase IstB	-
VVCECT4999_RS24280	58732..59232	-	501	WP_011988346.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	rtxE / rtxD / rtxB / rtxC	1..68446	68446

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (48-92)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11134.96 Da
• Isoelectric Point: 10.6229

>T61578 WP_011988339.1 NZ_CP014638:c54938-54639 [Vibrio vulnificus]
MIYNIKRTEIFNDWLRELKDPQARGSIAARVQRLTQGLNGDVKPVGAGISELRIHVGKGYRVYFKRSGKQIIVLLCGGNK
KTQNQDIEQAKLLAQELGL

Nucleotide

• Length: 300 bp

>T61578 NZ_CP014638:c54938-54639 [Vibrio vulnificus]
ATGATATATAATATCAAGCGCACCGAAATCTTTAATGATTGGCTGAGGGAGTTAAAAGACCCTCAAGCGCGAGGTTCAAT
CGCCGCGCGAGTGCAACGACTTACCCAAGGATTGAACGGAGACGTGAAACCTGTTGGTGCTGGTATCAGTGAACTCAGGA
TACATGTCGGTAAGGGTTATAGGGTTTACTTTAAGCGAAGCGGCAAGCAGATCATTGTTTTGCTGTGTGGCGGTAACAAG
AAAACTCAAAACCAAGATATTGAACAAGCAAAACTGCTTGCCCAAGAGCTTGGATTATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10453.90 Da
• Isoelectric Point: 4.6855

>AT61578 WP_011988338.1 NZ_CP014638:c54563-54267 [Vibrio vulnificus]
MSKNTAMLADFDPAEYLDTPEAIQAYLDEAVASGDAAFISQSLGVIARAKGMTELAEQTGLSRESLYRTLSQKGNPNFKG
FMAIISALGISLKLNVHA

Nucleotide

• Length: 297 bp

>AT61578 NZ_CP014638:c54563-54267 [Vibrio vulnificus]
ATGTCTAAAAATACAGCTATGCTCGCGGATTTTGACCCCGCAGAGTATCTTGATACCCCTGAAGCTATACAGGCGTATCT
CGATGAAGCTGTAGCCTCTGGTGATGCAGCTTTTATCAGTCAATCTCTCGGTGTGATTGCTCGCGCTAAGGGTATGACAG
AACTTGCAGAACAAACAGGACTATCCCGAGAATCGCTATATAGAACGCTTAGCCAAAAGGGAAACCCTAATTTTAAAGGA
TTTATGGCCATCATTTCAGCGTTGGGCATCTCTTTGAAATTGAACGTTCATGCTTAA

Structures

Toxin

• AlphaFold DB: A7DVA5