Detailed information of TA system TA061574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 1573766..1574264
• Replicon: chromosome
• Accession: NZ_CP014636
• Organism: Vibrio vulnificus strain CECT 4999

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: VVCECT4999_RS07925
• Protein ID: WP_000589156.1
• Coordinates: 1573766..1574032 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: VVCECT4999_RS07930
• Protein ID: WP_017420747.1
• Coordinates: 1574019..1574264 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VVCECT4999_RS24775	1568923..1569129	+	207	WP_080729784.1	DUF3265 domain-containing protein	-
VVCECT4999_RS07885	1569063..1569662	+	600	WP_157771840.1	hypothetical protein	-
VVCECT4999_RS07890	1569826..1570329	+	504	WP_088900033.1	hypothetical protein	-
VVCECT4999_RS07895	1570276..1571223	-	948	WP_011988311.1	IS30 family transposase	-
VVCECT4999_RS07900	1571358..1571843	+	486	WP_088900034.1	hypothetical protein	-
VVCECT4999_RS07910	1572192..1572599	+	408	WP_017420805.1	hypothetical protein	-
VVCECT4999_RS07915	1572606..1572728	+	123	WP_017420820.1	DUF3265 domain-containing protein	-
VVCECT4999_RS07920	1572749..1573621	+	873	WP_017420690.1	hypothetical protein	-
VVCECT4999_RS07925	1573766..1574032	+	267	WP_000589156.1	BrnT family toxin	Toxin
VVCECT4999_RS07930	1574019..1574264	+	246	WP_017420747.1	hypothetical protein	Antitoxin
VVCECT4999_RS07935	1574406..1574723	+	318	WP_039452835.1	heavy metal-binding domain-containing protein	-
VVCECT4999_RS07940	1574738..1574830	+	93	WP_193784089.1	DUF3265 domain-containing protein	-
VVCECT4999_RS07945	1574878..1575246	+	369	WP_026050325.1	VOC family protein	-
VVCECT4999_RS07950	1575733..1575861	+	129	Protein_1450	DUF3265 domain-containing protein	-
VVCECT4999_RS07955	1576206..1576787	+	582	WP_102938345.1	GNAT family N-acetyltransferase	-
VVCECT4999_RS07960	1576937..1577380	+	444	WP_017420781.1	GNAT family N-acetyltransferase	-
VVCECT4999_RS07965	1577313..1577530	+	218	Protein_1453	DUF3265 domain-containing protein	-
VVCECT4999_RS07970	1577535..1578116	+	582	WP_025796457.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1453300..1639357	186057
	-	inside	Integron	-	-	1531219..1607550	76331

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T61574 WP_000589156.1 NZ_CP014636:1573766-1574032 [Vibrio vulnificus]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T61574 NZ_CP014636:1573766-1574032 [Vibrio vulnificus]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCATGGTATTGATTTTCATACAGCTCAAGG
GTTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCGGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9477.78 Da
• Isoelectric Point: 6.8806

>AT61574 WP_017420747.1 NZ_CP014636:1574019-1574264 [Vibrio vulnificus]
MKAHEFDAKFESDDDDIVMNLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT61574 NZ_CP014636:1574019-1574264 [Vibrio vulnificus]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGAACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTGGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGTTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7