Detailed information of TA system TA061573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 1561467..1561965
• Replicon: chromosome
• Accession: NZ_CP014636
• Organism: Vibrio vulnificus strain CECT 4999

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: VVCECT4999_RS07785
• Protein ID: WP_000589156.1
• Coordinates: 1561467..1561733 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: VVCECT4999_RS07790
• Protein ID: WP_088900031.1
• Coordinates: 1561720..1561965 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VVCECT4999_RS07745	1556860..1556982	+	123	WP_080540714.1	DUF3265 domain-containing protein	-
VVCECT4999_RS07750	1556998..1557898	+	901	Protein_1415	hypothetical protein	-
VVCECT4999_RS07755	1558051..1558692	+	642	WP_045621783.1	hypothetical protein	-
VVCECT4999_RS07760	1558721..1558816	+	96	WP_080933183.1	DUF3265 domain-containing protein	-
VVCECT4999_RS07770	1560801..1560950	+	150	WP_088900030.1	DUF3265 domain-containing protein	-
VVCECT4999_RS07780	1561289..1561411	+	123	WP_102938339.1	DUF3265 domain-containing protein	-
VVCECT4999_RS07785	1561467..1561733	+	267	WP_000589156.1	BrnT family toxin	Toxin
VVCECT4999_RS07790	1561720..1561965	+	246	WP_088900031.1	CopG family transcriptional regulator	Antitoxin
VVCECT4999_RS07800	1562129..1562437	+	309	WP_017420768.1	hypothetical protein	-
VVCECT4999_RS07810	1562552..1562869	-	318	WP_017420671.1	CcdB family protein	-
VVCECT4999_RS07815	1562869..1563114	-	246	WP_017420672.1	type II toxin-antitoxin system CcdA family antitoxin	-
VVCECT4999_RS07820	1563171..1563293	+	123	Protein_1426	DUF3265 domain-containing protein	-
VVCECT4999_RS07825	1563312..1563782	+	471	WP_039452383.1	hypothetical protein	-
VVCECT4999_RS07830	1563769..1563915	+	147	WP_080729752.1	DUF3265 domain-containing protein	-
VVCECT4999_RS07840	1564265..1564393	+	129	WP_088900132.1	DUF3265 domain-containing protein	-
VVCECT4999_RS07845	1564738..1564860	+	123	Protein_1430	DUF3265 domain-containing protein	-
VVCECT4999_RS07850	1564890..1565363	+	474	WP_080532865.1	GNAT family N-acetyltransferase	-
VVCECT4999_RS07855	1565386..1566342	-	957	WP_039453501.1	IS110 family transposase	-
VVCECT4999_RS07860	1566539..1566706	+	168	Protein_1433	DUF3265 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1453300..1639357	186057
	-	inside	Integron	-	-	1531219..1607550	76331

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T61573 WP_000589156.1 NZ_CP014636:1561467-1561733 [Vibrio vulnificus]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T61573 NZ_CP014636:1561467-1561733 [Vibrio vulnificus]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
GTTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9492.79 Da
• Isoelectric Point: 6.4838

>AT61573 WP_088900031.1 NZ_CP014636:1561720-1561965 [Vibrio vulnificus]
MKAHEFDAKFESDDDEIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT61573 NZ_CP014636:1561720-1561965 [Vibrio vulnificus]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGAGATCGTAATGGACTTGGATCTATCACAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATAGGTGTAACACGTCAATCAATCATTAAAATTTGGCTTGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7