Detailed information of TA system TA061569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 1464948..1465486
• Replicon: chromosome
• Accession: NZ_CP014636
• Organism: Vibrio vulnificus strain CECT 4999

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A7X8U0Q7
• Locus tag: VVCECT4999_RS06830
• Protein ID: WP_019282621.1
• Coordinates: 1464948..1465247 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A7X8YM83
• Locus tag: VVCECT4999_RS06835
• Protein ID: WP_017045414.1
• Coordinates: 1465244..1465486 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VVCECT4999_RS06790	1460003..1460629	+	627	WP_088733245.1	nucleotidyltransferase domain-containing protein	-
VVCECT4999_RS06795	1460626..1460937	+	312	WP_088899994.1	hypothetical protein	-
VVCECT4999_RS06800	1461097..1461465	+	369	WP_088899995.1	VOC family protein	-
VVCECT4999_RS24550	1461531..1461602	+	72	WP_165399758.1	hypothetical protein	-
VVCECT4999_RS06810	1462718..1463113	+	396	WP_088899996.1	DUF3465 domain-containing protein	-
VVCECT4999_RS06820	1464168..1464260	+	93	WP_193784382.1	DUF3265 domain-containing protein	-
VVCECT4999_RS06825	1464298..1464830	+	533	Protein_1261	GNAT family N-acetyltransferase	-
VVCECT4999_RS06830	1464948..1465247	-	300	WP_019282621.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VVCECT4999_RS06835	1465244..1465486	-	243	WP_017045414.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
VVCECT4999_RS06840	1465570..1465662	+	93	WP_193784381.1	DUF3265 domain-containing protein	-
VVCECT4999_RS06845	1465705..1466694	+	990	WP_088899997.1	hypothetical protein	-
VVCECT4999_RS06850	1466721..1466813	+	93	WP_079854941.1	DUF3265 domain-containing protein	-
VVCECT4999_RS06855	1466866..1467471	+	606	WP_088899998.1	hypothetical protein	-
VVCECT4999_RS06860	1467504..1468532	+	1029	WP_011988369.1	IS630 family transposase	-
VVCECT4999_RS06865	1468868..1469170	+	303	WP_088899999.1	hypothetical protein	-
VVCECT4999_RS06870	1469316..1470203	+	888	WP_088900000.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1453300..1639357	186057
	-	inside	Integron	-	-	1452095..1525749	73654

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-92)

Antitoxin

• Antitoxin ParD (4-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11662.38 Da
• Isoelectric Point: 9.8917

>T61569 WP_019282621.1 NZ_CP014636:c1465247-1464948 [Vibrio vulnificus]
MKPFQLTNKAKSDLKDIALFTSRRWGREQRNIYLKQFDESFWLLAENPDIGKTCDEIRDGYRKFPQGSHVIFYQQIGSQN
IQIIRILHKSMDVNPIFGA

Nucleotide

• Length: 300 bp

>T61569 NZ_CP014636:c1465247-1464948 [Vibrio vulnificus]
ATGAAACCATTCCAACTAACCAACAAGGCAAAATCCGATTTAAAAGATATTGCCTTATTTACCTCTCGCAGGTGGGGCCG
CGAGCAACGAAATATTTATCTCAAACAATTCGATGAATCATTTTGGCTACTCGCTGAAAATCCTGATATCGGTAAAACTT
GTGATGAAATCAGAGATGGCTACCGAAAATTCCCACAAGGAAGCCATGTTATTTTTTATCAACAAATTGGTAGCCAAAAC
ATCCAGATCATAAGAATCTTGCATAAGAGTATGGATGTAAATCCAATCTTTGGCGCATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8947.89 Da
• Isoelectric Point: 4.2213

>AT61569 WP_017045414.1 NZ_CP014636:c1465486-1465244 [Vibrio vulnificus]
MAKNTSITLGDHFDGFISSQIQSGRYGSASEVIRSALRLLETQETKMNTLRQLLVEGEESGMADYDLDSFISELDSEERK

Nucleotide

• Length: 243 bp

>AT61569 NZ_CP014636:c1465486-1465244 [Vibrio vulnificus]
ATGGCTAAAAATACTAGCATCACTCTCGGCGACCACTTTGATGGTTTCATTAGTAGCCAAATCCAAAGTGGACGCTATGG
CTCAGCAAGTGAAGTAATTCGTTCCGCTCTACGTTTACTCGAAACACAAGAAACGAAAATGAACACTTTACGACAACTGC
TCGTTGAAGGTGAAGAAAGTGGTATGGCTGATTATGACCTCGATTCGTTTATCAGTGAACTAGATAGCGAAGAAAGAAAA
TGA

Structures

Toxin

• AlphaFold DB: A0A7X8U0Q7

Antitoxin

• AlphaFold DB: A0A7X8YM83