Detailed information of TA system TA061568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PanAT/DUF4065(antitoxin)
• Location: 152404..154079
• Replicon: chromosome
• Accession: NZ_CP014636
• Organism: Vibrio vulnificus strain CECT 4999

Toxin (Protein)

• Gene name: panT
• Uniprot ID: -
• Locus tag: VVCECT4999_RS00775
• Protein ID: WP_032473081.1
• Coordinates: 152868..154079 (+)
• Length: 404 a.a.

Antitoxin (Protein)

• Gene name: panA
• Uniprot ID: A0A0X8LPP8
• Locus tag: VVCECT4999_RS00770
• Protein ID: WP_000649312.1
• Coordinates: 152404..152871 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VVCECT4999_RS00740	147603..147824	-	222	WP_039450991.1	AlpA family transcriptional regulator	-
VVCECT4999_RS00745	147902..148891	+	990	WP_039450988.1	50S ribosome-binding GTPase	-
VVCECT4999_RS00750	149002..149448	+	447	WP_039450986.1	hypothetical protein	-
VVCECT4999_RS00755	149489..150133	-	645	WP_039450984.1	inovirus Gp2 family protein	-
VVCECT4999_RS00760	150300..151424	-	1125	WP_039450981.1	hypothetical protein	-
VVCECT4999_RS00765	151479..152150	+	672	WP_039450979.1	hypothetical protein	-
VVCECT4999_RS00770	152404..152871	+	468	WP_000649312.1	DUF4065 domain-containing protein	Antitoxin
VVCECT4999_RS00775	152868..154079	+	1212	WP_032473081.1	RelA/SpoT domain-containing protein	Toxin
VVCECT4999_RS00780	154325..155434	-	1110	WP_039450973.1	tRNA (uridine(54)-C5)-methyltransferase TrmA	-
VVCECT4999_RS00785	155837..157678	+	1842	WP_038941548.1	TonB-dependent receptor	-
VVCECT4999_RS00790	157723..158406	+	684	WP_180982276.1	adenine nucleotide alpha hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RelA/SpoT (86-226)

Antitoxin

• Antitoxin SocA-like, Panacea domain (32-123)

Sequences

Toxin

Protein

• Length: 404 a.a.
• Molecular weight: 45807.63 Da
• Isoelectric Point: 5.2762

>T61568 WP_032473081.1 NZ_CP014636:152868-154079 [Vibrio vulnificus]
VSDKEKRVVSFQIDDTPTAEQASKNFQDDYELCFQDFLERTVDESAAEDFSYSNNGVKKAGRRLRKKEGDLKSATATIQQ
FRAAHEKPLNTIAYLVGRCCRELGIDVKPVTRLKRLETIVDKLQRKSLDGETTNATCVTNMNDIGGCRAIFPDLESLEKA
KERLQVTVEKVGRIKIKDIDDYIANPKPNDCGYRSLHVIYRYDHASGKSFNIEAQLRTRLQHLWATTVEIVDILEGTKIK
THSHSPDEDKEKLQVQWEELLSIMSQYIADAEGAIELSNDNRTQFSNRLIELNREINALSRLRSFKILSEKVEGCRDGSI
EHVLLVIDETSLEMIFSEEFENYTQAISVYNAAEKMTRSHARLNTLLISTKNMGQLSEAYPNYLGDCASFITLLEDAMQS
GQN

Nucleotide

• Length: 1212 bp

>T61568 NZ_CP014636:152868-154079 [Vibrio vulnificus]
GTGAGTGATAAAGAAAAACGTGTCGTCAGTTTTCAAATTGACGACACTCCCACCGCTGAACAGGCATCAAAAAACTTCCA
AGACGACTATGAGCTGTGCTTCCAAGATTTTCTTGAAAGAACGGTTGATGAAAGTGCCGCTGAAGATTTCTCATACTCCA
ATAACGGAGTTAAGAAAGCAGGGCGACGACTACGCAAGAAAGAAGGCGATCTTAAATCCGCGACTGCGACCATCCAGCAG
TTTCGTGCTGCCCATGAAAAACCACTGAATACTATCGCCTACCTTGTTGGTCGATGTTGTCGAGAGCTTGGGATAGATGT
TAAACCAGTGACACGACTGAAGCGTTTAGAAACCATAGTAGATAAGCTACAGCGTAAATCTCTTGATGGTGAAACTACGA
ATGCGACCTGTGTAACCAATATGAACGATATTGGTGGTTGTCGGGCAATTTTCCCTGATCTTGAATCGTTGGAGAAAGCA
AAAGAGCGCCTGCAAGTTACCGTAGAAAAAGTAGGTCGAATTAAGATTAAGGACATTGATGACTACATCGCTAATCCTAA
ACCTAATGACTGCGGATACCGAAGTTTGCATGTTATATATCGTTATGACCATGCCAGCGGTAAAAGTTTTAATATAGAAG
CTCAGCTACGTACTCGTCTTCAGCATCTTTGGGCGACAACAGTAGAAATCGTCGATATTTTGGAAGGTACTAAGATTAAG
ACGCATTCCCATAGCCCAGATGAAGACAAAGAGAAGTTGCAGGTTCAGTGGGAAGAGCTACTTTCCATCATGAGTCAGTA
TATTGCTGACGCCGAAGGTGCTATAGAGTTAAGCAATGACAATAGGACTCAGTTTTCAAATAGACTGATTGAATTGAATC
GTGAAATCAATGCATTGAGTCGGTTGAGAAGCTTTAAAATCTTATCTGAAAAAGTTGAAGGCTGTCGTGATGGCTCAATA
GAACATGTTCTGTTAGTTATTGACGAAACTTCGTTAGAAATGATTTTTAGTGAAGAGTTTGAGAATTATACCCAAGCTAT
CTCGGTTTATAACGCAGCTGAGAAAATGACTCGTAGTCATGCGAGATTAAACACCCTTCTTATATCTACGAAAAATATGG
GGCAACTATCAGAAGCATACCCCAACTATCTAGGTGACTGTGCTTCATTCATCACTCTGCTTGAAGACGCTATGCAATCG
GGCCAGAATTAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17581.02 Da
• Isoelectric Point: 7.5659

>AT61568 WP_000649312.1 NZ_CP014636:152404-152871 [Vibrio vulnificus]
MKAYHTYSAVDVAWSLLKHAKEQGKCFSNLQLQKLTYVCHGLSLAHFQRPLVVDDVFAWKFGPVVPSVYFRFKSYGSNVI
TEQCDVVLDHESESIVKDVVSQLGHLTGPQLVDLTHREGSPWHQVWDGTHQKVIPDHLIQAHYTQIKQSGRTASL

Nucleotide

• Length: 468 bp

>AT61568 NZ_CP014636:152404-152871 [Vibrio vulnificus]
ATGAAAGCATATCACACATACAGTGCTGTAGACGTTGCATGGAGCTTGCTTAAGCATGCGAAAGAGCAAGGTAAATGCTT
CTCCAATTTGCAGCTACAAAAACTTACCTATGTTTGCCATGGTTTATCATTGGCACACTTCCAGCGTCCTCTTGTTGTGG
ATGACGTATTTGCTTGGAAATTTGGTCCCGTAGTCCCTTCTGTCTATTTTCGCTTTAAATCGTATGGCAGTAACGTGATC
ACCGAACAATGTGATGTTGTGTTAGATCATGAAAGTGAATCTATTGTCAAAGATGTAGTATCACAACTGGGGCATTTAAC
AGGCCCGCAGCTTGTAGATCTGACTCACCGTGAGGGTAGTCCATGGCATCAAGTATGGGATGGTACACATCAAAAAGTGA
TTCCTGACCATTTGATTCAAGCTCATTACACTCAGATAAAACAATCAGGGCGAACAGCTAGCCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0X8LPP8