Detailed information of TA system TA061541

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4340064..4340689
• Replicon: chromosome
• Accession: NZ_CP014583
• Organism: Escherichia coli strain CFSAN004176

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BCV59_RS23215
• Protein ID: WP_000911330.1
• Coordinates: 4340291..4340689 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: BCV59_RS23210
• Protein ID: WP_000450524.1
• Coordinates: 4340064..4340291 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCV59_RS23180	4335867..4336337	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
BCV59_RS23185	4336337..4336909	-	573	WP_069357523.1	glycine cleavage system transcriptional repressor	-
BCV59_RS23190	4337055..4337933	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
BCV59_RS23195	4337950..4338984	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
BCV59_RS23205	4339197..4339910	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
BCV59_RS23210	4340064..4340291	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
BCV59_RS23215	4340291..4340689	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
BCV59_RS23220	4340836..4341699	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
BCV59_RS23225	4341714..4343729	+	2016	WP_069357524.1	tRNA cytosine(34) acetyltransferase TmcA	-
BCV59_RS23230	4343803..4344501	+	699	WP_069357525.1	esterase	-
BCV59_RS23235	4344611..4344811	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T61541 WP_000911330.1 NZ_CP014583:4340291-4340689 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T61541 NZ_CP014583:4340291-4340689 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT61541 WP_000450524.1 NZ_CP014583:4340064-4340291 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT61541 NZ_CP014583:4340064-4340291 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33