Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3894549..3895203 Replicon chromosome
Accession NZ_CP014583
Organism Escherichia coli strain CFSAN004176

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag BCV59_RS21005 Protein ID WP_000244777.1
Coordinates 3894796..3895203 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag BCV59_RS21000 Protein ID WP_000354046.1
Coordinates 3894549..3894815 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCV59_RS20975 3889718..3890461 + 744 WP_000951948.1 SDR family oxidoreductase -
BCV59_RS20980 3890518..3891951 - 1434 WP_032359762.1 6-phospho-beta-glucosidase BglA -
BCV59_RS20985 3891996..3892307 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
BCV59_RS20990 3892471..3893130 + 660 WP_000250274.1 hemolysin III family protein -
BCV59_RS20995 3893326..3894306 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
BCV59_RS21000 3894549..3894815 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
BCV59_RS21005 3894796..3895203 + 408 WP_000244777.1 toxin CptA Toxin
BCV59_RS21010 3895243..3895764 - 522 WP_001055874.1 flavodoxin FldB -
BCV59_RS21015 3895876..3896772 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
BCV59_RS21020 3896796..3897506 + 711 WP_024226239.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
BCV59_RS21025 3897512..3899245 + 1734 WP_024226240.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T61538 WP_000244777.1 NZ_CP014583:3894796-3895203 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T61538 NZ_CP014583:3894796-3895203 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT61538 WP_000354046.1 NZ_CP014583:3894549-3894815 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT61538 NZ_CP014583:3894549-3894815 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References